Reagent Blood Collection Tube Additives Blood Coagulant For Hematology Analysis

Blood Collection Tube Reagent Vacuum Additive Blood Coagulant for Blood Testing Hematology Analysis

Serum is one of the main specimens tested in clinical biochemical and immunological tests. At present, the methods of obtaining serum samples in medical institutions are mainly obtained by centrifugation after collecting venous blood after the blood is completely coagulated. Under normal circumstances, it takes more than 60 minutes for an isolated blood sample to completely coagulate, or even not coagulate, which is difficult to meet the needs of rapid laboratory testing. Therefore, shortening the coagulation process and quickly separating serum samples are important factors to improve the efficiency of laboratory medicine. The accelerator was born from this.

Procoagulant principle of blood coagulant:

Activate part of the coagulation factors in the plasma and platelets, promote the formation of thromboplastin and fibrin clots, accelerate the contraction of blood clots, and achieve the purpose of rapid serum separation.

In vacuum blood collection tubes containing coagulant, fibrin precipitated filaments and lumps sometimes appear, which is caused by the unstandardized use of coagulation blood collection tubes. Choosing a coagulant with too fast coagulation speed in the preparation of vacuum blood collection tubes will cause fibrin to shrink too fast and fragile red blood cells will easily fragment and cause mild hemolysis. Generally, the precipitation of fibrin has the following reasons:

Use procoagulant blood collection tube or separation gel to promote coagulation blood collection tube. In order to make the coagulant evenly distributed in the blood, it must be slightly inverted and mixed 4 to 5 times to make the center of the blood collection tube and the surrounding blood coagulate at the same time. If the blood is not completely coagulated and then centrifuged, it can cause the precipitation of fibrin. The upper part of the serum appears jelly-like and fragmented, which is mixed with the blood. Fibrin filaments are caused by the fact that fibrin has not yet fully contracted.

Secondly, when the blood collection tube company prepared the coagulant blood collection tube, the coagulant was not sprayed enough or the prepared water-soluble coagulant was not used up on the same day, and continued use the next day, resulting in a decrease in the effectiveness of the coagulant. The fibrinogen in the blood is gradually transformed into insoluble fibrin under the action of the coagulant. If the coagulant dose is insufficient or fails to extend the clotting time, centrifugation can lead to the precipitation of fibrin.

Procoagulant principle of blood coagulant:

Activate part of the coagulation factors in the plasma and platelets, promote the formation of thromboplastin and fibrin clots, accelerate the contraction of blood clots, and achieve the purpose of rapid serum separation.

In vacuum blood collection tubes containing coagulant, fibrin precipitated filaments and lumps sometimes appear, which is caused by the unstandardized use of coagulation blood collection tubes. Choosing a coagulant with too fast coagulation speed in the preparation of vacuum blood collection tubes will cause fibrin to shrink too fast and fragile red blood cells will easily fragment and cause mild hemolysis. Generally, the precipitation of fibrin has the following reasons:

Use procoagulant blood collection tube or separation gel to promote coagulation blood collection tube. In order to make the coagulant evenly distributed in the blood, it must be slightly inverted and mixed 4 to 5 times to make the center of the blood collection tube and the surrounding blood coagulate at the same time. If the blood is not completely coagulated and then centrifuged, it can cause the precipitation of fibrin. The upper part of the serum appears jelly-like and fragmented, which is mixed with the blood. Fibrin filaments are caused by the fact that fibrin has not yet fully contracted.

Secondly, when the blood collection tube company prepared the coagulant blood collection tube, the coagulant was not sprayed enough or the prepared water-soluble coagulant was not used up on the same day, and continued use the next day, resulting in a decrease in the effectiveness of the coagulant. The fibrinogen in the blood is gradually transformed into insoluble fibrin under the action of the coagulant. If the coagulant dose is insufficient or fails to extend the clotting time, centrifugation can lead to the precipitation of fibrin.

Specification:

[precautions]

1. Blood coagulation is related to environmental temperature, reverse mixing degree, blood characteristics of patients, coagulant performance and blood volume, especially with temperature. When the ambient temperature of blood collection is lower than 20 ℃, the dosage of coagulant should be increased properly, the times of reverse mixing should be increased, the coagulation time should be prolonged, or it should be used together with 36 ℃ water bath box.

2. Blood cells and fibrin filaments will hang on the wall and slightly hemolysis will occur when coagulation is not sufficient. In some patients, when the blood is not completely clotted at one time, the clotting process may be stopped, collagen may precipitate or there is no serum separation after centrifugation.

3. The coagulation time is 5-15 minutes, that is, centrifugation on the machine, which may lead to the secondary separation of fibrin filaments and the appearance of fibrin filaments floating in the serum, which is very easy to cause the blockage of the probe.

4. When accelerator and separator are used at the same time, poor coagulation effect will affect serum quality and separator isolation effect.

5. After adding to the blood vessel for irradiation and sterilization, it is recommended to use γ - ray irradiation with a dose of 8 ~ 250kgy. The irradiation dose was controlled by the initial colony number.

6. It is recommended not to touch the coagulant directly by hand. When it is stuck to the skin, it can be rinsed with water.

[warning and prevention]

When it is found that there are impurities, abnormal smell, abnormal color and expiration date in the accelerator, it is forbidden to use it; this product is organic solvent liquid, with definite smell, inflammable, slight anesthetic performance, non-toxic.