The chromogenic substrate MAOS is fully known as N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate, CAS No. 82692-97-5.Like other new Trinder's reagents, it has high water solubility and much higher sensitivity than traditional color reagents commonly used in some laboratories, so it is widely used in clinical detection and biochemical kits.
||N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate
MAOS chromogenic substrate
Firstly, the biggest characteristic of MAOS is that the maximum absorption wavelength of its oxidized product is far more than that of common color reagents. Even in the new Trinder's reagent, the ultraviolet absorption wavelength of its product is 630 nm, which is quite high.The maximum absorption wavelength of products of many chromogenic reagents is in the visible region. If it is used to detect human blood or other body fluids, due to the complexity of the components in the sample to be tested, the sample itself contains a small amount of components whose absorption wavelength is in the visible region or similar to the absorption wavelength of the chromogenic products, which will make the detection results higher.If, like MAOS, the maximum absorption wavelength of the product is in the ultraviolet region and is relatively high, the interference will be greatly reduced.Therefore, MAOS is recommended as a chromogenic substrate for some biochemical detection items requiring highly accurate values.
Secondly, the color reaction of MAOS chromogenic substrate has a wide range of adaptability to the pH of the reaction system, which greatly improves its adaptability.Some chromogenic reagents often react under acidic conditions, but the participation of enzymes is usually required in biochemical detection. We know that the catalytic action of enzymes is relatively sensitive to the pH of the reaction environment, and can not match with the chromogenic reagents or even inactivate them, which will be greatly limited in use, while MAOS reagents do not have this problem.
In addition, the molar absorbance of MAOS color reaction is relatively high, so its sensitivity is also relatively high.It should be noted that MAOS will fade when the detection time is too long, so MAOS detection needs to be completed in time and can not be interrupted.In addition, MAOS is also relatively safe, unlike biphenyl reagents such as DAB, although the price is relatively low, it has certain carcinogenicity or mutagenicity.
Desheng Technology has been committed to the research and development, production and sales of various chromogenic substrates, especially TOOS, MAOS, ADPS and so on, which have a good reputation at home and abroad, and has a good cooperative relationship with many biochemical kit manufacturers, jointly contributing to the domestic field of diagnostic reagents!