Preparation Of Biological Buffer Trihydroxymethylaminomethane Purity Above 99%

Tris buffer, also known as trimethylaminomethane, is a commonly used biochemical laboratory buffer that is widely used to regulate the acid-base balance of experimental systems. Its simple preparation, stable pH performance, and widespread application in multiple fields make it an indispensable tool in the laboratory. So how was it prepared?

Tris parameters:

Tris Property

Tris is an organic base with the molecular formula C4H11NO3 and a molecular weight of 121.135 g/mol. It is a white crystalline solid, highly soluble in water and weakly alkaline. In biochemical experiments, tris buffer is often used to adjust the pH value of the solution to maintain it within a specific range to meet the needs of the experiment.

Preparation method of tris buffer

A common method for preparing tris buffer is to dissolve trimethylaminomethane in distilled water and adjust the pH value using an acid such as hydrochloric acid. The preparation process should pay attention to the following points:

1. Firstly, weigh the required amount of trihydroxymethylaminomethane powder, then slowly pour it into distilled water and stir to dissolve it. The concentration of the solution can be selected according to experimental requirements, usually within the range of 10 mM to 100 mM.

2. Add acid (such as hydrochloric acid) dropwise to the solution while monitoring the changes in pH value using a pH meter. According to the experimental requirements, adjust the pH value to the desired range. The pH range of tris is usually between 7.0 and 9.0, but it can also be adjusted according to the needs of the experiment.

3. The prepared tris buffer can be filtered or sterilized to obtain the final product, ensuring the accuracy and reliability of the experiment.

tris powder

Application of tris buffer

Tris buffer has a wide range of applications in biochemical experiments, roughly as follows:

1. Nucleic acid electrophoresis: In nucleic acid electrophoresis experiments such as DNA and RNA, tris can be used as an electrophoresis buffer to help maintain a constant pH value during the electrophoresis process and ensure separation efficiency.

2. Protein electrophoresis: In protein electrophoresis experiments, it can be used as a buffer for the preparation and operation of gel, which is helpful for protein separation and analysis.

3. Enzyme activity experiment: In the enzyme activity experiment, pH value is one of the important factors affecting enzyme activity. Tris buffer can adjust the pH value of the experimental system, enabling enzymes to exert maximum activity under the most suitable pH conditions.

4. Cell culture: Tris can be used in the process of cell culture to regulate the pH value of the culture medium and provide a suitable growth environment for cells.

5. Protein crystallization: In protein crystallization experiments, it can also be used to adjust the pH value of the solution to obtain suitable conditions for protein crystallization.

Tris buffer has become an indispensable and important reagent in biochemical laboratories due to its stable performance and wide application fields. By using a simple preparation method, a buffer solution with the required concentration and pH value can be obtained, which is suitable for multiple fields and provides strong support for the accuracy and reliability of experimental results. As an advantageous manufacturer of biological buffering agents, Desheng can provide affordable and stable tris. If you are interested, please feel free to contact us at any time!