Glutamate dehydrogenase (GLDH) for quantitative determination of biological samples in vitro

In the field of life sciences, every enzyme is like a "code" for cellular activity, and glutamate dehydrogenase (GLDH) is undoubtedly one of the key keys to unlocking the secrets of liver health. As a member of the zinc metal binding enzyme family, GLDH is mainly distributed in mitochondria of tissues such as liver, kidney, and myocardium, and its activity changes directly reflect the cellular metabolic status and organ function damage. With the rise of precision medicine, the application of GLDH in quantitative measurement in vitro is moving from the laboratory to the clinical front line, becoming an important tool for evaluating liver health, monitoring drug hepatotoxicity, and studying metabolic diseases.

The detection principle and technological breakthrough of GLDH

GLDH participates in energy metabolism by catalyzing the deamination reaction of glutamic acid, and its activity detection is based on the dynamic changes in the absorbance of coenzyme NADH in enzymatic reactions. Modern detection technology has broken through the limitations of traditional methods: using biochemical methods combined with ELISA kits, precise quantification of multiple sample types such as serum, plasma, urine, liver tissue, and cell culture supernatant has been achieved. It is worth emphasizing that the GLDH detection kit demonstrates significant advantages - the liquid stable ready to use reagent does not require reconstitution, and when combined with an automated analysis system, it not only minimizes detection errors, but also has the ability to resist jaundice and hemolysis interference, providing reliable detection guarantees for clinical use.

glutamic dehydrogenase

Clinical value: a "specificity scale" for the diagnosis of liver disease

GLDH is becoming a novel biomarker that complements traditional indicators in the diagnosis of liver diseases. Its core values are reflected in three aspects:

1. Organizational specificity: GLDH mainly exists in the mitochondria of liver cells. When liver cells are damaged, mitochondria rupture and release GLDH into the bloodstream, with an increase much greater than ALT/AST. Research shows that GLDH in patients with cirrhosis can increase to 21.84 times the normal value, while AST only increases by 7.17 times.

2. Damage localization ability: By calculating the ratio of ALT+AST/GLDH, liver disease types can be identified. For example, in patients with acute viral hepatitis, the ratio is greater than 50, while in alcoholic hepatitis, the GLDH elevation in the central lobular area (GLDH enrichment zone) is 1.3 times that of ALT due to specific damage.

3. Advantages of dynamic monitoring: GLDH activity fluctuates in real-time with the degree of liver injury. In drug-induced liver injury (DILI), its level changes earlier than ALT/AST, providing a critical time window for clinical intervention.

Technological Empowerment: The 'All rounder' for Multi scenario Applications

The universality of GLDH detection technology is reshaping research paradigms in multiple fields:

Drug development: As an FDA approved biomarker for drug-induced liver injury, GLDH can accurately monitor drug hepatotoxicity in clinical trials. For example, in the development of anti tuberculosis drugs, the combined detection of GLDH and inflammatory factors has increased the specificity of liver injury diagnosis by 40%.

Basic scientific research: In metabonomics research, GLDH activity reflects the efficiency of amino acid metabolic pathway, providing a new perspective for the study of metabolism related diseases such as diabetes and obesity.

Forensic identification: Inferring the time of death and degree of liver injury by measuring the GLDH content in bodily fluids, providing auxiliary evidence for case investigation.

Company Building

Hubei Xindesheng Material Technology Co., Ltd. has established a professional enzyme preparation research and development team, and has successfully developed glutamate dehydrogenase, uricase, adenosine deaminase, cholesterol oxidase, etc. If you need this type of product, please click on the official website to learn more details or contact me!