Recommended storage buffer for glutamate dehydrogenase 9029-12-3: TRIS hydrochloride

In the field of biochemistry research and enzyme preservation, glutamate dehydrogenase (GLDH) is a key metabolic enzyme whose activity and stability directly affect the accuracy of experimental results. It is crucial to choose a suitable buffer system to ensure that the enzyme maintains its optimal state during storage. Among numerous buffer solutions, TRIS hydrochloride has become the preferred solution for the preservation of glutamate dehydrogenase due to its excellent chemical properties and wide applicability.

TRIS hydrochloride: a "pH stabilizer" for biological experiments

TRIS hydrochloride (Tris HCl) is a classic biological buffering agent, whose core advantage lies in its precise pH regulation ability. Its pKa value is close to the physiological range (about 8.1), and the effective buffering range covers pH 7.0 to 9.0, providing a stable acid-base environment for enzyme molecules. This characteristic makes it widely used in experiments such as DNA/RNA extraction, protein purification, and enzyme activity determination. For example, in the gel electrophoresis experiment, TRIS buffer solution can ensure that the pH in the electric field is constant, and avoid the degradation of nucleic acid or protein due to acid-base changes, thus ensuring the repeatability of the experimental results.

glutamate dehydrogenase

Ideal habitat for glutamate dehydrogenase: pH matching and stability assurance

The activity of glutamate dehydrogenase is highly dependent on the pH value of the solution, and its optimal pH range is 7.0 to 8.0. The buffer interval of TRIS hydrochloride exactly overlaps with this, which can effectively resist pH fluctuations caused by external factors such as temperature changes and air contact by dynamically balancing H ⁺ and OH ⁻ ions in the solution. Research has shown that when enzymes deviate from their optimal pH range, their spatial conformation may undergo irreversible changes, leading to loss of activity. TRIS buffer can significantly prolong the half-life of enzymes and reduce activity loss caused by acid-base imbalance by maintaining pH stability.

The delicate balance between ion strength and enzyme activity

The ionic strength of buffer directly affects the solubility and conformational stability of enzymes. TRIS hydrochloride has a low ionic strength, which can maintain the osmotic pressure of the solution without causing enzyme molecule aggregation or denaturation due to high salt concentration. For enzymes such as glutamate dehydrogenase that are sensitive to salt concentration, low ionic strength TRIS buffer can reduce non-specific interactions, ensure the natural conformation of the enzyme, and maintain its catalytic activity.

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TRIS hydrochloride, with its precise pH buffering ability, low-temperature stability, low ionic strength, and safety, has become an ideal buffer for the preservation of glutamate dehydrogenase. In biochemical research, selecting appropriate buffer solutions is not only a technical issue, but also a commitment to experimental rigor. Hubei Xindesheng Material Technology Co., Ltd. has established a professional enzyme preparation research and development team, and has successfully developed glutamate dehydrogenase, uricase, adenosine deaminase, cholesterol oxidase, etc. If you need this type of product, please click on the official website to learn more details or contact me!