Relying on freeze-dried powder storage method to strengthen the defense line of IVD diagnostic enzyme activity

In the field of IVD diagnosis, the activity of diagnostic enzymes is the core to ensure accurate detection results, and the freeze-dried powder form, as a common storage form of diagnostic enzymes, directly determines whether the diagnostic enzymes can maintain stable activity for a long time. Only by accurately grasping the key points of freeze-dried powder storage methods can a solid "protective barrier" be built for IVD diagnostic enzymes, ensuring their optimal performance in detection applications.

Core point one: Strict low-temperature control to prevent loss of activity

The primary core of the preservation method for freeze-dried powder is strict low-temperature control. The molecular structure of IVD diagnostic enzyme freeze-dried powder is extremely sensitive to temperature changes, and high temperature environments can accelerate the denaturation process of enzyme proteins, resulting in irreversible loss of activity. Therefore, during the storage process, unopened freeze-dried powder needs to be placed in a frozen environment below -20 ℃. For some special diagnostic enzyme freeze-dried powder with higher temperature requirements, the storage environment needs to be further upgraded, and temperature fluctuations caused by unstable refrigeration in the refrigerator need to be avoided. When taking freeze-dried powder from a frozen environment, the principle of "quick take, quick use" should be followed. If it cannot be used immediately, it should be returned to the frozen environment within 10 minutes to prevent prolonged exposure to room temperature from damaging enzyme activity. At the same time, it is necessary to eliminate repeated freezing and thawing operations, which can cause ice crystals to form inside the freeze-dried powder and disrupt the spatial structure of enzymes. It is recommended to divide the freeze-dried powder into small sizes according to the single use amount before the first use, and the packaging process should be carried out on a sterile operating table. After packaging, it should be immediately sealed and frozen to avoid damage to enzyme activity from repeated freezing and thawing at the root.

Adenosine deaminase

Core point 2: Maintain a dry environment and prevent deliquescence and agglomeration

Maintaining a dry environment is another key step in the preservation of freeze-dried powder. IVD diagnostic enzyme freeze-dried powder has extremely strong hygroscopicity. Once it comes into contact with humid air, it will quickly absorb moisture and cause deliquescence and agglomeration, leading to the aggregation of enzyme molecules and a significant decrease in activity. When storing, it is necessary to strictly control the humidity of the environment. A hygrometer can be placed in the storage cabinet for real-time monitoring. If the humidity exceeds the range, the dehumidification equipment should be turned on in a timely manner for adjustment. Unopened freeze-dried powder packaging is usually equipped with silica gel desiccant. The desiccant should be retained and the packaging should be sealed properly. If any damage or leakage is found in the packaging, a new sealed packaging should be replaced immediately to prevent moisture from entering. For opened freeze-dried powder, seal it as soon as possible after each use. Parafilm sealing film can be used to reinforce the seal and prevent moisture from leaking into packaging gaps. At the same time, the desiccant inside the packaging should be replaced every 3 months to ensure a continuous and stable drying effect.

Core point three: Take good measures to avoid light and standardize the access process

In addition, the storage method of freeze-dried powder also needs to be accompanied by comprehensive light avoidance measures and standardized retrieval procedures. Some active ingredients in IVD diagnostic enzyme freeze-dried powder are sensitive to light, especially ultraviolet and strong light, which can cause oxidation reactions of enzyme molecules and damage active sites. Therefore, the storag e location should be chosen in a cool and dark place, avoiding direct sunlight or close to strong light sources. A shading board can be pasted inside the storage cabinet, or a non transparent container can be used to store the freeze-dried powder. During the retrieval operation, operators should wear sterile gloves and masks to prevent contamination of freeze-dried powder by hand sweat and respiratory moisture; Access tools such as pipette tips and medicine spoons must undergo high-temperature sterilization treatment and be specially replaced. Cross use is prohibited; During the retrieval process, the freeze-dried powder should not be exposed to air for more than 2 minutes. After retrieval, it should be immediately sealed and returned to the designated storage environment to reduce external interference with enzyme activity.

Summary: Multi link collaboration to safeguard diagnostic enzyme activity

The preservation method of freeze-dried powder is a key carrier to ensure the activity of IVD diagnostic enzymes. From low temperature control to dry maintenance, from light protection to standardized use, every step is interrelated and indispensable. Only by implementing the various requirements for the storage of freeze-dried powder in practical operations can we effectively delay the decline of IVD diagnostic enzyme activity, extend its service life, provide strong support for the accuracy of in vitro diagnostic testing, and assist medical institutions in providing more reliable diagnosis and treatment services for patients. ​

Desheng Company

Hubei Xindesheng Material Technology Co., Ltd. has always adhered to the production and operation philosophy of "quality first, technology leadership" and "innovation and pursuit of excellence", continuously researching and developing reagents needed in the diagnostic field. Since its establishment in 2005, it has a history of nearly 20 years and is deeply trusted and supported by users. The diagnostic enzyme reagents independently developed and produced by Desheng have been sold to various parts of the world. If you have any related procurement needs in the near future, please feel free to contact us!