Scientific storage of lactate dehydrogenase 9028-36-8: why it must be packaged and avoid repeated freeze-thaw cycles

Lactate dehydrogenase (LDH), as a key enzyme in the glucose metabolism pathway of organisms, is widely present in various tissues, cells, and microorganisms of the human body, and plays an irreplaceable role in clinical diagnosis, scientific research experiments, and other fields. Whether it is a clinical kit for detecting myocardial injury or a laboratory reagent for studying cell metabolism, the stability of LDH activity directly determines the accuracy of experimental results and product quality. However, in practical use, the decrease in enzyme activity caused by improper storage methods often becomes a hidden obstacle that affects detection accuracy and experimental reproducibility. Among them, packaging storage and avoiding repeated freezing and thawing are the two core points to ensure LDH activity, which contain rigorous biochemical principles and practical experience.

1, Molecular structure perspective: Why repeated freeze-thaw cycles can damage LDH activity

To understand why special attention should be paid to the storage method of LDH, it is necessary to first clarify its molecular structural characteristics. LDH is essentially a oligomeric enzyme composed of four subunits, which are connected by non covalent bonds to form a stable spatial conformation, which is the basis for the active center of the enzyme to function. When the enzyme solution is in a low-temperature frozen state, water molecules will form regular ice crystal structures. The mechanical force generated during the ice crystal growth process will disrupt the spatial conformation of the enzyme molecules, leading to subunit dissociation or deformation of the active center. If the entire bottle of LDH is repeatedly taken out of the -20 ℃ or -80 ℃ refrigerator and melted, and then frozen again after use, this "freezing melting" cycle will continue to exacerbate the damage to the molecular structure - the first freezing thawing will cause some enzyme molecules to lose activity, and each subsequent freezing thawing will further damage the structure of the remaining active enzymes, ultimately leading to a significant decrease in the overall activity of the enzyme solution, or even complete inactivation.

Lactate Dehydrogenase

2, Practical application risk: specific hazard verification of repeated freeze-thaw cycles

From the perspective of practical application scenarios, the negative impact of repeated freeze-thaw cycles on LDH has been validated by a large amount of experimental data. In clinical diagnosis, using repeated freeze-thaw LDH reagents to detect serum samples may result in low or false negative test results, delaying the diagnosis of diseases such as myocardial infarction and liver disease; In scientific research experiments, the instability of enzyme activity can lead to excessive fluctuations in experimental data, making it difficult to form reproducible conclusions and wasting a lot of time and sample resources. It is worth noting that LDH enzyme solution usually contains buffering agents, stabilizers, and other components. Repeated freeze-thaw cycles can disrupt the uniformity of these components, further accelerating the loss of enzyme activity.

3, Scientific solution: Operational specifications for LDH packaging and storage

In response to the above issues, packaging and storage have become key measures to ensure LDH activity. When performing specific operations, LDH should be quickly divided into sterile, enzyme free centrifuge tubes or reagent bottles according to the required amount for each use after the first thawing. The packaging process should be carried out under ice bath conditions to avoid prolonged exposure of the enzyme solution to room temperature; The volume of each dispensing bottle should be used up in one go to avoid the remaining enzyme solution from freezing and thawing again. After the packaging is completed, the enzyme solution should be immediately placed in a -20 ℃ (short-term storage, 1-3 months) or -80 ℃ (long-term storage, more than 6 months) freezer for freezing storage. At the same time, the name, concentration, packaging date, and expiration date of the enzyme should be clearly labeled on the container for subsequent management and use.

4, Detail supplement: Precautions for LDH retrieval and thawing

In addition, during the retrieval and thawing process of LDH, the following details should be noted: the enzyme solution taken out of the refrigerator should be slowly thawed in an ice bath, avoiding direct melting at room temperature or heating in a microwave oven; The thawed enzyme solution should be gently mixed to avoid severe shaking that may cause denaturation of enzyme molecules; If multiple bottles of enzyme solution need to be used for one experiment, they should be thawed separately to avoid repeatedly freezing and thawing unused enzyme solution due to taking out multiple bottles at the same time.

5, Pay attention to storage management and ensure the application value of LDH

In summary, the stability of lactate dehydrogenase activity is closely related to its storage method, and repeated freezing and thawing are the main factors leading to a decrease in enzyme activity. Scientific packaging and storage are effective means to ensure enzyme activity. Whether it is clinical testing institutions, biopharmaceutical companies, or research laboratories, they should attach great importance to the storage management of LDH, strictly follow the packaging operation specifications, avoid repeated freezing and thawing, to ensure the accuracy of experimental results and the stability of product quality, and provide reliable guarantees for research and application in related fields.

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Hubei Xindesheng Material Technology Co., Ltd. has always adhered to the production and operation philosophy of "quality first, technology leadership" and "innovation and pursuit of excellence", continuously researching and developing reagents needed in the diagnostic field. Since its establishment in 2005, it has a history of nearly 20 years and is deeply trusted and supported by users. The diagnostic enzyme reagents independently developed and produced by Desheng have been sold to various parts of the world. If you have any related procurement needs in the near future, please feel free to contact us!