Detailed explanation of core parameters and technical indicators of N - (2-acetylamino) iminodiacetic acid buffer

1.Basic Product Information

ADA (N - (2-acetamido) iminodiacetic acid) is a widely used zwitterionic biological buffer in the fields of biochemistry and molecular biology. It belongs to the "Good's buffer" family developed by Dr. Norman Good and his colleagues in the 1960s.

2.Core buffering performance parameters

2.1  Buffer range and pKa value

The effective buffering range of ADA is pH 6.0-7.2. At 25 ℃, its pKa value is 6.6. At 20 ℃, the pKa value is approximately 6.1-7.1.

This buffer range covers the optimal pH range for numerous enzymatic reactions and cell culture processes, enabling it to stably maintain the acid-base environment of the reaction system in various biological experimental scenarios.

2.2  pKa temperature coefficient

The pKa temperature coefficient (Δ pKa/° C) of ADA is -0.011. This low value means that its pKa value fluctuates less during temperature changes. This feature enables ADA to maintain relatively stable buffering performance during transportation, storage, and experimental processes, especially suitable for applications with frequent temperature changes.

2.3  pH value of aqueous solution

The pH value of ADA in pure water is 1.5-3.0 (25 ℃, 0.01M aqueous solution). The pH range of its 1% aqueous solution at 25 ℃ is 4.6-6.0.

3.Purity and quality indicators

3.1  Purity

The purity of high-quality ADA products can reach ≥ 99.0% (determined by titration). Some ultra pure products can achieve a purity of ≥ 99.0% (T). High purity is a key indicator to ensure stable buffer performance and minimal inter batch differences.

3.2  Dry weight loss (moisture)

The drying weight loss of high-quality ADA products is usually controlled at ≤ 0.5%. Some product specifications are ≤ 1.0%. Low moisture content helps ensure the long-term stability and weighing accuracy of the product.

3.3 Burning residue

The burn residue of high-quality ADA products is usually ≤ 0.1%.

3.4 Heavy metal content

ADA products of different levels have strict requirements for heavy metal content:

Advanced pure/ultra pure grade: Heavy metals (calculated as Pb) ≤ 3-5ppm

Biochemical grade: Heavy metals (calculated as Pb) ≤ 5-10 ppm

4.Solubility parameters

ADA is soluble in water and ethanol. The specific solubility properties are as follows:

Solubility can reach 160 mg/mL in 1M NaOH

The solubility in water is 2.64 g/L

It can be prepared into a 10% aqueous solution in hot water, which is transparent and colorless

0.05M aqueous solution can be prepared as a clear and colorless solution

ADA has better solubility in hot water than in cold water, and can be heated appropriately during preparation to accelerate dissolution. It should be noted that ADA is slightly soluble in water and insoluble in organic solvents such as ethanol .

5.Spectral characteristics

ADA has low ultraviolet absorption at wavelengths below 260nm. The specific UV absorption indicators are as follows:

Absorption at 260nm (0.05M aqueous solution): ≤ 0.20

Absorption at 280nm (0.05M aqueous solution): ≤ 0.05

Absorption at 290nm (10%, 1N NaOH): ≤ 0.15

The low UV absorption characteristics make ADA particularly suitable for analytical experiments that require UV detection, effectively avoiding interference from the buffer itself on the detection signal.

6.Other important parameters

6.1 Metal ion chelating ability

ADA has excellent metal ion chelating properties and can chelate metal ions such as manganese (II), copper (II), nickel (II), zinc (II), cobalt (II) in a ratio of 2:1. This characteristic makes it uniquely valuable in experimental systems that require control of metal ion concentration.

6.2  Refractive index

The refractive index of ADA is approximately 1.4957-1.5010.

6.3 Storage conditions

ADA should be stored in a sealed environment at room temperature, away from light, and dry. It is recommended to store some high-purity products in a cool and dark environment at<15 ° C.

7. Hubei Xindesheng - a professional supplier of high-quality ADA buffering agents

Hubei Xindesheng Material Technology Co., Ltd. has been deeply involved in the field of biological buffering agents for nearly 20 years, and has a complete research and development, production, and quality control system for ADA biological buffering agents. The purity of ADA products produced by the company can reach over 99%, and all technical indicators are strictly benchmarked against international standards. Xindesheng has established a full process quality control system from raw material inspection to finished product inspection, ensuring stable and reliable technical indicators for each batch of products. The company can also provide customized services according to customer needs to meet the differentiated requirements for ADA buffer quality in different application scenarios.

If you need to obtain specific product COA (Certificate of Analysis) or learn more technical details, please contact Hubei Xindesheng Material Technology Co., Ltd. through the official website.