Analysis of the dosage and usage of serum separation gel

Serum separation gel is a key material for achieving physical isolation between serum and blood cells in blood collection vessels. In actual production, the amount and operation process of separation gel directly affect the separation effect and stability of blood collection tubes. Different specifications of blood collection tubes correspond to different amounts of additives, and there are clear sequential requirements for the processing steps after adding glue.

The dosage of separation gel for different specifications of blood collection tubes

There are various sizes of blood collection tubes, ranging from 2 milliliters to 9 milliliters, with tube diameters of 13 millimeters and 16 millimeters. The amount of separation gel added is not strictly proportional to the volume of the blood collection tube. The blood collection tubes of 2ml, 3ml, and 4ml sizes all use 13 × 75mm tubes, and the amount of separation gel added is 0.8g. The 5ml and 6ml specifications use 13 × 100mm tubes with the same addition amount of 0.8g. The 9ml specification uses a 16 × 100mm tube body, and the addition amount is increased to 1g.

From these data, it can be seen that for a tube with a diameter of 13 millimeters, whether the volume is 2 milliliters or 6 milliliters, the addition of 0.8 grams of separation gel can basically meet the usage requirements. The key is that the final thickness of the separation adhesive layer needs to reach 6 millimeters, which is the minimum requirement for effective isolation. Regardless of the total volume of the blood collection tube, the separation gel after centrifugation should form a continuous barrier of sufficient thickness between serum and blood cells to prevent the exchange of substances between the two sides.

Process flow after adding separation adhesive

The addition of separation gel is not the final step in the production of blood collection tubes, there are multiple processing steps that follow. The standard procedure is to add glue first, and then leave it for 24 hours. The purpose of this static step is to ensure that the separation glue is fully leveled and stable at the bottom of the tube, eliminating internal stress or uneven distribution generated during the gluing process. After placement, add the coagulant. The function of coagulants is to accelerate the coagulation process after blood collection and shorten the sample processing time.

After adding the coagulant, a drying operation is required to remove excess moisture from the tube. Subsequently, vacuum is applied to create a negative pressure environment inside the blood collection tube, which facilitates the automatic flow of blood into the tube during blood collection. Finally, there is the packaging process. The produced blood collection tubes need to undergo centrifugation validation during use, with centrifugation parameters generally set at 3000 revolutions per minute for 15 minutes. Under these conditions, the separation gel should be able to migrate smoothly between serum and blood cells, forming a complete isolation layer.

The core use of separation glue

The main function of serum separation gel is to form an isolation layer between the cellular components of blood and serum. This physical barrier can prevent the exchange of substances between blood cells and serum, including the diffusion of potassium ions, enzymes, and other components from blood cells into serum, as well as the absorption of certain components in serum by blood cells. By preventing these exchanges, the separation gel ensures that the blood sample maintains its original characteristics for a certain period of time, making the test results more accurate and reliable.

At present, the application scenario of separation gel is mainly for the examination of human blood samples. Meanwhile, with the development of veterinary testing and animal experiments, separation gels have also been used for the extraction and examination of animal blood samples. In addition, by utilizing the precise specific gravity characteristics of the separation gel, various specific cellular components or analytes can be extracted from the blood.

Hubei Xindesheng Material Technology Co., Ltd. has been deeply involved in the field of serum separation gel for many years. With professional research and strict production, we have created products with excellent performance and stable quality. We always uphold a rigorous and responsible attitude, providing high-quality serum separation gel for the medical testing industry. Choosing Hubei Xindesheng means choosing a professional, stable, and reliable medical testing guarantee. If you have any purchasing needs in the near future, please click on the official website to learn more details!