Payment & Shipping Terms:
CAS 3682-14-2 Chemiluminescent Reagent,
What are the production processes of isoluminol
Isoluminol is also known as 4-aminophthalic hydrazide. It is a white powder with a CAS number of 3682-14-2. Luminol is a commonly used luminol chemiluminescence reagent. Its luminescence mechanism is oxidation reaction luminescence. It is catalyzed by horseradish peroxidase under alkaline conditions and oxidized by H2O2 to produce 4-aminophthalic acid. The excited state intermediate of, when it returns to the ground state, it will emit photons. Commonly used in food, environment, criminal investigation, medicine and other fields. The following will introduce several methods of making isoluminol.
1. Using phthalic anhydride as raw material, reacting with urea to prepare phthalimide product; then, under the catalysis of concentrated sulfuric acid and fuming nitric acid, 4-nitro-phthalimide is prepared Amine; then add hydrazine hydrate to react to prepare 4-nitro-phthalic hydrazide; then in an alkaline solution environment, with stannous chloride solution as a catalyst, the target product isoluminol is prepared by catalysis. The synthesis process of this method is simple, and the reaction operation is simple. However, stannous chloride is used as a catalyst to catalyze the reaction, and a large amount of inorganic tin waste will be generated, which causes great environmental pollution and does not meet the requirements of green synthesis.
2. Using phthalic hydrazide as raw material, react with acetylation reagent to obtain diacetyl phthalic hydrazide; then react with nitroso compound to obtain nitroso diacetyl phthalic hydrazide; Then through deacetylation reaction to obtain nitrosophthalic hydrazide; and then through reduction reaction to obtain isoluminol. This method makes full use of the large amount of phthalic hydrazide by-products in the existing Gabriel amine synthesis method as raw materials, and the raw materials are cheap and easy to obtain. At the same time, the synthesis process is simple and the yield of isoluminol products is high, but it has advantages It is also its disadvantage. The by-product of Gabriel amine is used as a raw material, and its quality is unstable, and the batch difference is large. In addition, the intermediate nitroso compound in the preparation process is more toxic and has potential safety hazards, which is not suitable for large-scale industrial production. demand.
3. First, prepare phthalimide, then react it with concentrated sulfuric acid and fuming nitric acid, and obtain 4-nitrophthalimide after cooling and suction filtration. Then the preparation of 4-nitrophthalic hydrazide is mixed with a certain concentration of hydrazine hydrate and water and refluxed to obtain an orange-red solution. After cooling, adjust the pH with glacial acetic acid, and obtain by precipitation, washing, suction filtration, and drying. Light yellow solid 4-nitrophthalic acid hydrazide; finally 4-nitrophthalic acid hydrazide is warmly dissolved in sodium hydroxide solution, and the solution is dripped to contain 56g of stannous chloride under constant stirring Add nano-titanium dioxide to the pure solution of concentrated hydrochloric acid, and then react under ultraviolet light to obtain a colorless and clear solution. After cooling, precipitation, suction filtration, precipitation and suction filtration, etc., it is then dried to obtain a white powdery solid It is isoluminol; this method has complicated synthesis steps and is not suitable for industrial production, but has the advantages of high yield and high purity of isoluminol obtained.
|Melting point||300℃||Boiling point||633ºCat760mmHg|
Hubei New Desheng Materials is a high-tech enterprise specializing in the production and development of blood collection tube additives, chemiluminescence reagents, biological buffers, chromogen substrates, enzyme preparations, carbomers and other products. The company has been established for decades, and has many years of product development and production. It has an independent research and development team and has rich experience in product production and research and development. At present, the products produced by Desheng have been sold to many countries around the world, all of which have received favorable comments and repurchases, and have reached cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.
Contact Person: Sales Manager