Payment & Shipping Terms:
|Appearance:||Yellow Powder||Molecular Formula:||C8H7N3O2|
CAS 521-31-3 Luminol Blood Test,
C8H7N3O2 Luminol Blood Test,
CAS 521-31-3 luminol testing fluid
How does luminol perform luminescence detection
Luminol is also called luminol, and its full name is 3-aminophthalic hydrazide. Luminol is widely used as a chemiluminescence reagent and is often used in chemiluminescence immunoassay biochemical systems. The appearance is yellow crystal or beige powder at room temperature, and it is a relatively stable chemical reagent. It is often used for blood testing in criminal investigation scenes. When luminol reagent is sprayed on the blood, it will oxidize with active oxygen and release blue-violet fluorescence, which is called luminol reaction. How does luminol perform luminescence detection, and how many detection methods are there? Many people still don’t understand, this article will introduce Luminol's luminescence detection method.
Adding catalyst is a commonly used method at present. Under normal circumstances, the chemiluminescence reaction of hydrogen peroxide and luminol luminescence system is particularly slow, but after adding certain catalysts, the reaction becomes particularly rapid.
At present, the main catalysts of luminol include some metal complexes and transition metal ions. The metal complexes mainly include hemoglobin and peroxidase; the transition metal ions mainly include Fe3+, Fe2+, Mn2+, Cr2+, etc. The catalyst that we often use in chemiluminescence immunoassay is peroxide, especially horseradish peroxidase. Some compounds can be immunoreacted with peroxidase-labeled antibodies, and then chemiluminescence assays can be performed with luminol to determine these compounds or antibodies. Such as digoxin, hepatitis B surface antigen, etc. are all detected and analyzed by this method.
In addition to the catalyst’s acceleration of detection, some inhibitors were also found in the research. These organic compounds can inhibit the chemiluminescence of luminol, such as reducing compounds containing phenolic hydroxyl groups, which can react with oxidants during the reaction, reducing the oxidants. The concentration of, the intensity of chemiluminescence is reduced, so as to indirectly measure this kind of organic substances.
Another method is to perform indirect determination through a coupling reaction. The coupling reaction here refers to combining a reaction that can produce or consume a chemiluminescent reactant with another chemiluminescent reaction, so as to achieve indirect chemiluminescence of certain substances. Determination. For example, some substrates produce hydrogen peroxide under the action of certain enzymes and then produce chemiluminescence with luminol. By measuring chemiluminescence, the purity of the measured substrate can be indirectly known.
Hubei New Desheng Materials specializes in the production and development of chemiluminescence reagents, luminescent substrates, biological buffers, blood collection tube additives and enzyme preparations. Desheng has been established for decades and has its own R&D team. It has been researching and developing chemiluminescence reagents for many years. It has many kinds of raw chemical products under its umbrella. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.
Contact Person: Sales Manager