Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White crystal powder
Keywords: Good’s buffer, PIPES, piperazine-1,4-diethanesulfonic acid
The full name of PIPES is piperazine-1,4-diethanesulfonic acid, a substance that can maintain the pH of the solution within a certain range. When a certain amount of acid and alkali is added to some solutions, it has the effect of hindering the pH change of the solution, which is called a buffering biological buffer. Such a solution is called a buffer solution. PIPES is an important member of Good’s buffer. Good’s buffer is a buffer system dedicated to life science research, which does not participate in and does not interfere with biochemical processes.
||White crystalline powder
||Room temperature, light-proof and moisture-proof
Application of PIPES
It can not form stable complexes with most metal ions and is suitable for buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox system. Low concentration of PIPES buffer solution should be used in cation exchange chromatography because PIPES has relatively high ionic strength and its pKa value is concentration dependent.
Advantage of PIPES
The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.
Hubei New Desheng Technology Co., Ltd. was founded in 2005, specializing in the research, development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. In terms of blood collection tube additives, it has formed its own intellectual property rights and professional production research and development capabilities. Provide products and raw material solutions for more than 100 domestic and foreign manufacturers.
At present, the Good buffer solutions supplied by our company include Tris, Bicine, CAPS, MOPS, TAPS, EPPS, etc .,all of which is white crystals, purity> 99%, absorbance <0.05. Welcome enterprises or individuals to call for details.
Preparation method of PIPES solution (about 1-2L) with different pH value
1. With 0.1M solution (A)
(1) NaOH/deionized water=4g/400ml …①
(2) "①+PIPES 30.237g"...②
(3) ②+Deionized water (about 600ml)=1,000ml
2. With 0.1M NaOH solution (B): NaOH 4g/ deionized water 1,000ml
3.pH 5.6 1,000ml(A)+0ml(B) (A):(B)=5:0
pH 6.4 1,000ml(A)+200ml(B) (A):(B)=5:1
pH 6.8 1,000ml(A)+400ml(B) (A):(B)=5:2
pH 7.2 1,000ml(A)+600ml(B) (A):(B)=5:3
pH 7.7 1,000ml(A)+800ml(B) (A):(B)=5:4
Structure diagram of piperazine-1,4-diethanesulfonic acid (PIPES)
The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine). It cannot form stable complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. Since PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.