Home
Products
About Us
Factory Tour
Quality Control
Contact Us
Request A Quote
News
News
Wuhan Desheng Biochemical Technology Co., Ltd
Home ProductsBlood Collection Tube Additives

Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder

China Wuhan Desheng Biochemical Technology Co., Ltd certification
China Wuhan Desheng Biochemical Technology Co., Ltd certification
As a distributor of hospital agent , your Blood Collection Tube Additives is very suit for my needs , i think we have establish a good business with each other , thank you !

—— Tony

I'm Online Chat Now

Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder

Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder
Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder

Large Image :  Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White Powder

Product Details:
Place of Origin: EZHOU, CHINA
Brand Name: DESHENG
Certification: ISO9001:2008
Model Number: HEPES
Payment & Shipping Terms:
Minimum Order Quantity: 10g
Price: Negotiable
Packaging Details: Plastic bottle/Ctn Box
Delivery Time: 1-3 days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 1000kg/Month
Detailed Product Description
Name: HEPES Full Name: Piperazine-1,4-Diethanesulfonic Acid
Category: Good's Buffer Function: PH Adjustment
CAS No.: 5625-37-6 Purity: >99%
Appearance: White Crystal Powder Molecular Weight: 302.368
Molecular Formula: C8H18N2O6S2 Industry: Medical & Health
High Light:

PIPES Good's Buffer

,

Cas 5625-37-6 Good's Buffer

,

cas 5625-37-6 PIPES Buffer

Cas 5625-37-6 PIPES Good's Buffer Piperazine-1,4-Diethanesulfonic Acid White crystal powder

 

Keywords: Good’s buffer, PIPES, piperazine-1,4-diethanesulfonic acid

The full name of PIPES is piperazine-1,4-diethanesulfonic acid, a substance that can maintain the pH of the solution within a certain range. When a certain amount of acid and alkali is added to some solutions, it has the effect of hindering the pH change of the solution, which is called a buffering biological buffer. Such a solution is called a buffer solution. PIPES is an important member of Good’s buffer. Good’s buffer is a buffer system dedicated to life science research, which does not participate in and does not interfere with biochemical processes.

 

Basic information

Product name Piperazine-N,N'-bis(2-ethanesulfonic acid)
Abbreviation PIPES Molecular formula C8H18N2O6S2
CAS No 5625-37-6 Molecular weight 302.40
Appearance White crystalline powder Purity >99%
Storage Conditions Room temperature, light-proof and moisture-proof

 

Molecular Structure

哌嗪-N,N'-双(2-乙磺酸)结构式

 

Application of PIPES

It can not form stable complexes with most metal ions and is suitable for buffer in solution system containing metal ions. It was applied to the purification of tubulin by using cellulose phosphate chromatography. The purified recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration and used as buffer to crystallize ketoenzyme from E. coli. In addition, because PIPES can form free radicals, it is not suitable for redox system. Low concentration of PIPES buffer solution should be used in cation exchange chromatography because PIPES has relatively high ionic strength and its pKa value is concentration dependent.

 

Advantage of PIPES

The purity (> 99%) is water-soluble, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.

 

Hubei New Desheng Technology Co., Ltd. was founded in 2005, specializing in the research, development, production and sales of blood collection tube additives, in vitro diagnostic reagents, buffers, and luminescent substrates. In terms of blood collection tube additives, it has formed its own intellectual property rights and professional production research and development capabilities. Provide products and raw material solutions for more than 100 domestic and foreign manufacturers.
At present, the Good buffer solutions supplied by our company include Tris, Bicine, CAPS, MOPS, TAPS, EPPS, etc .,all of which is white crystals, purity> 99%, absorbance <0.05. Welcome enterprises or individuals to call for details.

Good Buffer Solutions PIPES CAS5625-37-6  White crystal powder Purity>99% 1

 

Preparation method of PIPES solution (about 1-2L) with different pH value

1. With 0.1M solution (A)

(1) NaOH/deionized water=4g/400ml …①

(2) "①+PIPES 30.237g"...②

(3) ②+Deionized water (about 600ml)=1,000ml

2. With 0.1M NaOH solution (B): NaOH 4g/ deionized water 1,000ml

3.pH 5.6 1,000ml(A)+0ml(B) (A):(B)=5:0

pH 6.4 1,000ml(A)+200ml(B) (A):(B)=5:1

pH 6.8 1,000ml(A)+400ml(B) (A):(B)=5:2

pH 7.2 1,000ml(A)+600ml(B) (A):(B)=5:3

pH 7.7 1,000ml(A)+800ml(B) (A):(B)=5:4

Good Buffer Solutions PIPES CAS5625-37-6  White crystal powder Purity>99% 2

Structure diagram of piperazine-1,4-diethanesulfonic acid (PIPES)

The pH buffer range of PIPES is 6.1-7.5, insoluble in water, soluble in NaOH aqueous solution. PIPES is different from buffers containing two (2-hydroxyethyl) amino groups (such as Bis-tris, Bicine). It cannot form stable complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. According to existing research results, PIPES can be used to purify tubulin using phosphocellulose chromatography, to purify recombinant GTP-binding proteins ARF1 and ARF2 by gel filtration, and to use as a buffer to crystallize transketolase from E. coli. Since PIPES can form free radicals, it is not suitable for redox systems. In cation exchange chromatography, a low concentration of PIPES buffer should be used, because PIPES has a relatively large ionic strength and its pKa value is concentration-dependent.

Good Buffer Solutions PIPES CAS5625-37-6  White crystal powder Purity>99% 3

Contact Details
Wuhan Desheng Biochemical Technology Co., Ltd

Contact Person: Josphe

Tel: +8615071057538

Send your inquiry directly to us (0 / 3000)