Blood Collection Tube Additives-EDTA K3 Anticogulant for Whole Blood Analysis
[product name] tri potassium EDTA dihydrate
[introduction] What is K3 EDTA?
K3 ETDA is an alternative form of K2 EDTA used during collecting blood for the routine hematological tests. In comparison to K2 EDTA, K3 EDTA contains three potassium ions bound to EDTA.
However, the International Council for Standardization in Haematology recommends K2 EDTA as the anticoagulant due to two reasons: the increasing EDTA concentration in K3 EDTA results in increasing the shrinkage of red blood cells (11% shrinkage with 7.5 mg/ml blood). and upon standing, K3 EDTA increases the cell volume largely.
Also, K3 EDTA lowers the MCV values up to -0.1 to -1.3% when compared to K2 EDTA. Another drawback of K3 EDTA is that it results in the dilution of the blood specimen. It lowers most of the measurements including RBC, WBC, platelet, and Hgb counts by 1-2%.
[Formula] edtak3 · 2H2O
[package specification] plastic bottle, 500g / bottle
[product performance] white crystal powder, odorless, soluble in water, easy to absorb moisture, molecular weight of 442.56, pH value of 7.3 ± 1 (5% aqueous solution, g / ml,), solubility in water ≥ 60% (g / ml, 25 ℃). Stable at room temperature. The main content of EDTA in the company is ≥ 99.0%. See the product test report for other indicators.
[background] the EDTA K3 Blood Collection Tube is available with a pink top, a lavender top or white top blood tube and is used primarily in hematology and blood banking. The inner wall of blood bank tube is coated with spray-dried K3 EDTA additive which acts as an anticoagulant, binding calcium ions and interrupting the clotting cascade.
[main use] this product is specially used for anticoagulation of blood in vitro. It is an anticoagulant additive for clinical blood test. It is used for blood sample pretreatment (anticoagulation treatment) in the process of clinical blood collection and test.
[scope of application] EDTA salt can protect the cell composition of blood, not affect the white blood cell count and size, has the least impact on the morphology of red blood cells, and can inhibit the aggregation of platelets, which is suitable for general hematology test. In addition to the separation and test of platelets, it is not suitable for coagulation test and platelet kinetic energy test, nor for the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test.
The International Committee for standardization of Hematology (ICSH) recommended EDTA as the anticoagulant for blood cell count and classification. Compared with EDTA, it has higher solubility and faster anticoagulation speed.
[warning and prevention]
When impurities, foreign matters, abnormal smell, color, serious moisture absorption and expiration date occur in EDTA, it is forbidden to use.
Do not continue to use EDTA Tripotassium solution in case of turbidity.
This product is a chemical reagent with strong corrosiveness. It is forbidden to touch it directly. When splashing on the skin, wash with plenty of water. Splashing into eyes can be washed with plenty of water immediately. In case of emergency, it should be sent to hospital for examination and treatment immediately.
[storage conditions] the product can be sealed and stored in a cool and dry place, away from light and moisture. The aqueous solution of this product can be sealed and stored in sterile state at room temperature. It is recommended that the aqueous solution of this product be prepared and used immediately, and not stored in solution state for a long time.
[term of validity] 3 years
||Blood collection tube additive
P.S.[What is the Difference Between K2 EDTA and K3 EDTA?]
The main difference between K2 EDTA and K3 EDTA is that K2 EDTA contains two chelated potassium ions whereas K3 EDTA contains three chelated potassium ions. Furthermore, K2 EDTA slightly increases the MCV (mean corpuscular volume) of red blood cells at higher concentrations while K3 EDTA has no influence on the MCV of red blood cells at higher concentrations.
K2 EDTA and K3 EDTA are two types of anticoagulants used in routine hematological tests. However, their influence on the blood count remains controversial.