Serum separating gelWhat are the requirements for blood sampling additives?
In the laboratory of a hospital, you can see various test tubes. These tubes are not only used to store blood, but also can serve as an auxiliary test. Why do you say that? This is because reagents are added to it, such as commonly used serum separation gel, EDTA dipotassium, heparin sodium, heparin lithium, etc. What are the requirements for the use or addition of these blood collection additives? Below is a detailed explanation for everyone.
1、 Serum separating gel
First of all, there are requirements for temperature during the use process. Due to low temperature and increased viscosity, it may be difficult to add glue. The recommended temperature range is between - 20 -+90 degrees. If it is too low, you can also use a water bath for heating treatment. In addition, there are also requirements for the amount of separation gel added to the test tube, with a standard addition amount of 0.8-1.2g. For a tube with a diameter of 16mm, the amount of gel added should be above 1.0-1.2g.
Serum separating gel
2、 Anticoagulant
There are many types of anticoagulants. Let's take heparin sodium, heparin lithium, and EDTA dipotassium as examples to highlight the following requirements.
1. Heparin sodium, heparin lithium
The requirement is mainly for use in test tubes. To ensure sufficient anticoagulation of blood, it is necessary to slowly invert and mix for 5-8 times immediately after the completion of blood collection. Especially when the blood collection environment is high, if insufficient anticoagulation is not possible, it can easily lead to local coagulation. In addition, due to its non irreversible anticoagulation, it is necessary to complete the test within 6 hours after blood collection, and cannot be placed on hold for a long time, otherwise the results are prone to errors.
2. EDTA dipotassium
The requirement in testing is that after collecting the blood sample, it should be inverted 5-8 times as soon as possible to prevent the formation of small blood clots. It is also non irreversible anticoagulation like heparin, so it is required to complete the test within 24 hours. In addition, there are also requirements for the amount of potassium EDTA added, adding 1.5-2.2 mg of potassium EDTA solution per milliliter of blood. When used with other reagents, the addition requirement is to add 1.2-2.0 mg of potassium EDTA solution per milliliter of blood.
3、 Coagulant accelerator
The main requirement is to ensure that the clotting time is around 5-15 minutes before centrifugation. Direct centrifugation is not allowed, which can easily cause fibrin filaments to precipitate, and the detection effect is poor. Moreover, there are requirements for the addition amount of coagulant promoting agents. The addition standard is 20-30 Ul, which can promote the coagulation of 2-5 ml of blood. If the ambient temperature is too low or the blood collection volume is too high, it is necessary to appropriately increase the addition amount of coagulant promoting agents.
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