With the rapid development of in vitro diagnostics (IVD) industry towards real-time and convenient direction, dry chemistry method has been increasingly widely used in the field of blood lipid detection due to its advantages of easy operation, small sample size, and fast results. As an important member of the new Trinder's reagent, MAOS (N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline sodium salt monohydrate) plays a crucial role in color development in this technical pathway.
1, Basic principles of dry chemical method for blood lipid testing
Dry chemistry method uses solid-phase reagent technology to solidify chemical reagents onto a carrier. Taking blood lipid testing as an example, when a blood sample is dropped onto the test strip sampling area, lipid components such as cholesterol and triglycerides in the sample are oxidized by corresponding enzymes, producing hydrogen peroxide (H ₂ O ₂). H ₂ O ₂ undergoes oxidative coupling reaction with chromogenic substrates under the catalysis of peroxidase (POD), generating colored dyes. The analyzer can quantitatively calculate the content of the analyte by detecting the color intensity of the reaction endpoint.
2, The mechanism of MAOS in blood lipid test strips
MAOS is a highly water-soluble derivative of aniline sodium salt, which participates in color development as a hydrogen donor in the Trinder reaction. Under the catalysis of peroxidase, MAOS undergoes oxidative coupling reaction with 4-aminoantipyrine (4-AAP) to produce a green blue colored substance. The intensity of this colorimetric reaction is directly proportional to the concentration of the target substance in the sample, which is the basis for achieving quantitative detection.
At present, there are multiple lipid testing products in China that use dry chemistry methods and incorporate MAOS into the core reaction system. For example, in the approved blood lipid multi assay kit (dry chemistry method), the reaction layers of high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), and total cholesterol (TCH) all contain MAOS (20mmol/L). The total cholesterol test strip (dry chemistry) also uses MAOS as the core color developing component. The market registration and clinical application of these products have verified the feasibility and reliability of MAOS in dry chemical blood lipid testing.
3, The technical advantages of MAOS applied to blood lipid test strips
The reason why MAOS can play a good role in blood lipid test strips is mainly based on the following technical characteristics:
High absorption wavelength effectively reduces interference. The maximum absorption wavelength of MAOS oxidation products can reach 630nm. The absorption wavelength of endogenous interfering substances such as bilirubin in blood samples is mainly concentrated in the visible light range of 380-530nm. The absorption wavelength of MAOS products precisely avoids this range, which can effectively reduce the interference of complex components in the sample on the detection results, especially suitable for blood lipid detection projects that require high accuracy.
High color sensitivity, suitable for trace detection. MAOS has a high molar absorption intensity and high detection sensitivity, and can quickly develop color under low concentration target conditions. This characteristic enables it to meet the sensitivity requirements of dry chemical methods for color reactions.
Wide pH adaptability range ensures enzyme activity. The color reaction of MAOS has a wide adaptability range to the pH of the reaction system. Blood lipid testing involves various enzymes such as cholesterol esterase, cholesterol oxidase, peroxidase, etc., and the activity of these enzymes is sensitive to the pH of the reaction environment. The wide pH adaptability of MAOS helps maintain the catalytic activity of various enzymes and ensures the accuracy of detection results.
High water solubility, suitable for solid phase carriers. As a highly water-soluble derivative of aniline, MAOS has good solubility. In the preparation process of dry chemical test strips, reagents need to be impregnated or coated on a carrier in solution form and dried and cured. The high water solubility of MAOS contributes to its uniform distribution and effective fixation on solid-phase carriers.
Hubei Xindesheng Material Technology Co., Ltd. is deeply engaged in the field of IVD reagent raw materials and has nearly 20 years of research and production experience. The purity of MAOS chromogenic substrate produced by the company is ≥ 99%, and the product appearance is pure white crystal powder with good water solubility and stable process. The product has been widely used in various types of in vitro diagnostic kits. The company has two R&D and production bases in Gedian and Huanggang (70 acres), with an annual production capacity of 5000 tons for all categories.
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