Dry Lithium Heparin blood Anticoagulant CAS NO.9045-22-1

In medical testing and clinical trials, anticoagulants are often used to prevent blood clotting. Heparin lithium, as a commonly used anticoagulant, enhances the inhibitory effect of antithrombin III on coagulation factors by binding to it, thereby preventing blood clotting. In order to ensure that heparin lithium can effectively exert anticoagulant effects in blood collection tubes, special attention needs to be paid to its configuration details.

Basic principles for the preparation of heparin lithium anticoagulant

1. Accurate concentration: The concentration of heparin lithium anticoagulant should be accurately configured according to different testing items and sample types. Excessive concentration may lead to excessive blood dilution, affecting test results; If the concentration is too low, it may not effectively prevent blood clotting. Generally speaking, the concentration of heparin lithium is equal to the weight of the corresponding heparin lithium (mg) divided by the volume of the additive (UL).

 heparin lithium powder

2. Uniform mixing: When preparing heparin lithium anticoagulant, it should be ensured that heparin lithium is fully dissolved and evenly mixed in the solution.

Uneven mixing may lead to insufficient anticoagulation in some blood samples, thereby affecting the experimental results. The dissolution and mixing of heparin lithium can be promoted through stirring, oscillation, or ultrasound.  

3. Aseptic operation: The preparation of heparin lithium anticoagulant should be carried out under sterile conditions to avoid bacterial contamination of blood samples. Using sterile containers, pipettes, and reagents, strictly following sterile operating procedures, can effectively reduce the risk of contamination.

4. Stability: The configured heparin lithium anticoagulant should have a certain degree of stability and maintain its anticoagulant effect during storage and use. Generally speaking, heparin lithium anticoagulant should be stored in a cool, dry place, away from direct sunlight and high temperature environments. Meanwhile, it should be used within the prescribed validity period, as expired anticoagulants may lose their anticoagulant effect.

Configuration requirements for different sample types

In the industry standard for vacuum blood collection tubes, the recommended dosage range is 9.4IU to 28IU units of lithium heparin per milliliter of blood. For anticoagulation with micro blood collection devices, it can be less than 14IU per milliliter of blood. Desheng Company recommends using 15-20 IU heparin lithium to anticoagulate one milliliter of blood, and 8-10 IU heparin lithium to anticoagulate one milliliter of blood for peripheral blood anticoagulation.

1. Whole blood sample: For whole blood sample collection, if heparin lithium anticoagulant from Desheng Company is used, it can be configured according to different situations. In general, adding 15-20 IU of lithium heparin per milliliter of blood can ensure good anticoagulant effect. After collecting blood, ensure that the blood is thoroughly mixed with anticoagulants.

2. Micro blood collection sample: For samples collected by micro blood collection devices, due to the small amount of blood collected, the use of heparin lithium can be appropriately reduced to less than 14IU per milliliter of blood to avoid excessive blood dilution affecting the test results.

3. Peripheral blood sample: When collecting peripheral blood, 8-10 IU of anticoagulant per milliliter of blood is used. During the collection process, attention should also be paid to thoroughly mixing the blood with anticoagulants to ensure their anticoagulant effect.

Precautions during the configuration process

1. Reagent quality: Choosing high-quality heparin lithium reagents is the key to ensuring accurate anticoagulant configuration. We should choose heparin lithium reagents produced by legitimate manufacturers with good quality assurance, and pay attention to checking the expiration date and potency of the reagents.  

2. Container selection: Sterile and pyrogen free containers should be used for configuring heparin lithium anticoagulant. Both glass and plastic containers can be used, but attention should be paid to selecting appropriate materials and specifications to avoid the adsorption and impact of containers on heparin lithium.  

3. Pipette calibration: Using an accurate pipette is an important step in ensuring accurate concentration of heparin lithium. The pipette should be calibrated regularly to ensure its accuracy and precision. When absorbing heparin lithium reagent, attention should be paid to avoiding errors.

4. Record and identification: When configuring heparin lithium anticoagulant, the time, concentration, batch, and other information of the configuration should be recorded, and the configured anticoagulant should be clearly identified. This can facilitate traceability and management, ensuring the accuracy of experimental results.

Product packaging

The preparation of heparin lithium anticoagulant is one of the important steps in medical testing and clinical trials. The correct configuration of heparin lithium anticoagulant requires following basic principles such as accurate concentration, uniform mixing, sterile operation, stability, etc., and adjusting according to different sample types. During the configuration process, attention should be paid to reagent quality, container selection, pipette calibration, recording, and labeling. Only by strictly following the configuration principles and requirements can accurate and reliable blood samples be provided for medical testing and clinical experiments.

As a manufacturer of heparin lithium anticoagulant raw materials, Desheng can provide heparin lithium with a potency of 150IU-180IU. It has low impurities, simple preparation and use, and good anticoagulant effect. It has cooperated with multiple enterprises and exported to multiple countries. If you have purchasing intentions, please feel free to click on the website to inquire about details at any time!