Solubility And Dissolution Method Of Tripotassium EDTA

Solubility and Dissolution Method of Tripotassium EDTA

Tripotassium ethylenediaminetetraacetate, also known as EDTA K3, is white crystalline powder in appearance, tasteless and odorless, and has high solubility. Because of its excellent performance, it can be widely added to vacuum blood collection tubes and used as blood anticoagulant. Due to the different process and index requirements of tripotassium EDTA produced by various manufacturers, its solubility and dissolution method are also slightly different, so next I will introduce the solubility and dissolution method.

1. Solubility and Dissolution Method of Tripotassium EDTA

Tripotassium EDTA produced by Desheng has a water solubility of ≥60% (g/ml) at room temperature of 25 degrees. The main dissolution method is to use deionized water for dissolution, and slowly pour the powder of tripotassium EDTA into the volumetric flask of deionized water during continuous stirring.

If you see powder remaining on the wall of the beaker mouth, you can use a washing bottle to carefully flush it into the bottle, pay attention to the special cleaning of the volumetric flask and beaker, and do not contain impurities or oil. During the whole dissolving period, the addition amount of the aqueous solution needs to be specially controlled, and a certain amount of solvent is reserved for the tripotassium EDTA powder adhering to the cleaning utensils later, so as to avoid the decrease of the concentration.

Tripotassium EDTA Parameters:

2. Preparation of Tripotassium EDTA Solution

Usually, the blood collection volume of a blood collection tube is 2ml, and the dose of tripotassium EDTA to be added is 0.02ml, then the mass of EDTA in 2ml of blood is 3.6mg (1.8mg/ml*2ml ). The concentration of tripotassium EDTA in 1ml solution volume is 180mg/ml (3.6mg/0.02ml). If 500ml of tripotassium EDTA solution is prepared, the amount of 90g is required.

3. Addition of Tripotassium EDTA Solution

The tripotassium EDTA solution is added to the vacuum test tube, and there are often two addition methods, which are machine addition or manual addition. Small batches will be added manually, and more careful, large batches will be selected for machine addition, which can improve the overall efficiency.

Desheng Biochemical has been engaged in the R&D, production and sales of blood collection tube additives. The purity of the EDTA potassium salt series products produced can be as high as 99%. It is a dihydrate, analytically pure grade, and its stability is stronger. It has formed independent knowledge in this regard. property. In addition, it can also be individually customized according to customer needs, with a complete range of products. If you have requirements for blood collection tube reagents, please visit our website !