4-Morpholineethanesulfonic CAS 4432-31-9 Biological Buffer

MES acid is a medium-azide heterocyclic compound, known as 4-Morpholineethanesulfonic Acid, which is soluble in polar solvents such as water, ethanol, methanol, but insoluble in non-polar solvents. the molecular structure of MES acid includes a morpholine ring and an ethanesulfonic acid group, which is an amphoteric buffer with good water solubility and buffering properties as a negatively charged functional group.

Basic information on the buffers of MES acids

MES acid has a wide range of applications in biology and chemistry experiments as a Good's buffer. Its basic buffer information is as follows:

Buffer name: MES Buffer

Chemical formula: C6H13NO4S

Molecular weight: 195.24

Buffer pH range: 5.5-7.7

Buffer working concentration: 10-100mM

Buffer preparation method: mixture of MES acid and MES sodium salt MES buffer can be used in various biological experiments such as immunoblotting, protein electrophoresis, cell culture, etc. It can also be used in chemical experiments, such as enzyme-catalyzed reactions, acid-base titrations, etc.

Scenarios for the use of MES acids

MES acid acts as a Good's buffer and can be effectively buffered in the pH range 5.5 - 7.7. Therefore, it is widely used in biology and chemistry experiments in the following scenarios:

1. Cell culture

In cell culture, MES buffer can be used to adjust the pH of the culture medium to keep the cells in an optimum growth environment. In addition, it can be used as a buffer for the preparation of cell lyophilisation solutions. It helps to maintain the integrity of the cells and is also used in the preparation of viral vaccines.

2. Immunoblotting

In immunoblotting experiments, MES buffer can be used to prepare electrophoresis buffers to maintain the stability and charge balance of proteins in electrophoresis.

MES

3. Buffered electrophoresis

In protein electrophoresis experiments, MES buffers can be used to prepare electrophoresis buffers to maintain the stability and charge balance of proteins in electrophoresis. Used to run polyacrylamide gel electrophoresis (PAGE) of RNA, DNA or protein samples.

4. Enzyme-catalysed reactions

In enzyme-catalysed reactions, MES buffers can be used to adjust the pH of the reaction solution to maintain the enzyme in an optimum working environment.

5. Acid-base titrations

In acid-base titrations, MES buffer can be used as a titration solution buffer to maintain a stable pH during the titration.

6. Protein purification: MES can be used in protein purification processes. It helps to solubilise proteins and also helps to elute proteins from the resin during the purification process.

7. Industrial dispersants and deinking agents

It is used as an active substance in liquid cleaning, as a medium foam mineral flotation agent, as a dispersant in dyestuffs, pigments, pesticides, leather degreasing, and as a deinking agent in printing.

8. Daily chemical raw materials

Used as a buffering agent in daily chemical products such as face wash, detergent and shampoo.

Notes on use:

1. 4-Morpholineethanesulfonic acid is a strong irritant and should be handled with care. It can cause severe skin and eye irritation.

2. Avoid inhalation of powder or spray. Use appropriate protective equipment such as face shield, goggles and gloves.

3. MES is hygroscopic and should be stored in a cool, dry place. It should be kept away from moisture as it may form lumps in the presence of water.

4. 4-Morpholinoethanesulfonic acid should be kept away from heat and light. Exposure to sunlight may cause degradation of the compound.

Advantages of MES acid production by Desheng Biochemistry

Desheng Biochemical is a company that produces MES acid with the following advantages:

1. Professional Research and Development Team

With a professional R&D team, Desheng Biochemistry is able to continuously develop high quality MES buffer products to meet the needs of our customers.

2. Advanced production technology

Desheng Biochemistry adopts advanced production technology to ensure the quality and stability of MES buffer.

3. Strict Quality Control System

Desheng Biochemical implements a strict quality inspection system to ensure that the quality of MES buffer products meets the standards and customer requirements.

4. Large-scale production capacity

With large scale production capacity, Desheng Biochemistry is able to meet the large demand of customers and also provide customized services.

As a Good's buffer, MES acid has a wide range of applications in biology and chemistry experiments. As a manufacturer of MES acid, De Sheng Biochemical has a professional R&D team, advanced production technology, strict quality control system and large scale production capacity to provide customers with high quality MES buffer products and personalized customization services.