Direct Sales From Chinese Factory HEPES Buffer Powder Cas 7365-45-9

4-Hydroxyethylpiperazine Ethanesulfonic acid (HEPES) is a commonly used biological buffer, CAS No. 7365-45-9, white powder in appearance, good water solubility, purity up to 99%, widely used in biochemical and molecular biology experiments. Due to its many unique advantages, it has become one of the ideal buffer solutions for researchers to use. What are the characteristics of HEPES buffer powder? Let's take a look together.

Characteristics of HEPES buffer

1. Good buffering performance: HEPES has good buffering performance and can stabilize the pH values in cell culture media, enzyme reactions, and other biochemical experiments within the physiological pH range (pH 6.8-8.2).

2. Properties that do not affect biological processes: In cell culture and biochemical experiments, it does not interfere with biological processes and has no adverse effects on cell growth and enzyme activity.

3. Low toxicity: It has low toxicity to organisms and cells, and is suitable for cell culture and experiments inside and outside of organisms.

HEPES parameters:

HEPES powder application

1. Cell culture: It is often used for the preparation of cell culture media, which can stabilize the pH value of the culture medium and provide a suitable environment for cell growth.

2. Enzyme reaction: As a weak acid, it is often used as a Zwitterion buffer and pharmaceutical intermediate in organic chemical industry. The decomposition ability of this substance can vary with temperature, but its decomposition constant does not change much with temperature, which can better maintain the structure and function of the enzyme at low temperatures.

3. For environmental analysis: This type of research is more industrial grade HEPES, used as binding buffer and eluent in cation exchange, and used for grinding in plant research and development.

4. Molecular biology experiment: It is widely used in molecular biology experiments such as PCR, DNA/RNA electrophoresis and Gel electrophoresis of proteins to provide suitable environment and buffer capacity.

5. In daily chemical products, it can promote the metabolism of melanin, help whiten, and unblock pores to improve acne and blackheads; Place the skin in a weak acidic environment, inhibit inflammation and bacterial production, and achieve a healthy skin condition.

hepes powder

How to use HEPES

Firstly, dissolve an appropriate amount of HEPES buffer powder in distilled water, adjust the pH value to the required range according to experimental requirements, and prepare a buffer solution with the required concentration. During use, it is important to ensure complete dissolution. A portion of the powder can be added to the solution and stirred. If the dissolution effect is good, it can be dissolved to a certain concentration as required.

HEPES buffer powder has many advantages and is widely used in various fields. As a professional manufacturer, Desheng has strict requirements for its production quality, all of which are analytical grade and can meet the needs of most people. If you are interested, please contact us to purchase!