Analytical Purity Grade Of Biological Buffer Trihydroxymethyl Aminomethane CAS 77-86-1

Tris, also known as Tris, is a commonly used buffer and reagent, which is widely used in biochemical, medical or industrial fields. The different application fields correspond to different levels of use, and each has its own characteristics. What are the characteristics of pure Tris buffer? In what aspects can it be applied? Below, we will give you a specific introduction around this aspect.

tris powder

Chemical properties

Tris, with the chemical formula C13H22NNaO5S, is an amino alcohol compound that appears as a white crystalline powder and is soluble in water and some organic solvents such as ethanol. And it has good buffering ability, stable pH under neutral conditions, and is commonly used in biological experiments to maintain the stability of reaction systems.

Purity analysis

Tris analytical purity usually refers to its purity reaching 99% or higher, suitable for precision experiments and high demand applications, such as use as pharmaceutical intermediates. High performance liquid chromatography (HPLC), gas chromatography (GC), infrared spectroscopy (IR), and other methods are generally used to determine the purity, impurity content, and structural characteristics of Tris.

Tris parameters:

Application area

1. Buffer: As an excellent buffer, it is widely used in biochemical and molecular biology experiments. It can stabilize the activity of cells and enzymes and maintain appropriate pH conditions.

2. DNA/RNA electrophoresis: Tris acid EDTA (TAE) buffer or Tris boric acid EDTA (TBE) buffer is a commonly used DNA/RNA electrophoresis buffer for the separation and analysis of DNA/RNA fragments.

3. Protein research: It can also be used as a dissolving, purifying and stabilizing agent for proteins, and is often used in experiments such as Gel electrophoresis of proteins and immunoblotting.

4. Cell culture: It can be used for the preparation of cell culture media and the regulation of pH value, providing a suitable environment for cells.

5. Chemical synthesis: Tris can also be used as a catalyst or reducing agent for some organic synthesis reactions.

package

Precautions for use

Tris analysis is highly hygroscopic and should be stored in a dry environment to avoid moisture. At the same time, it should be sealed and stored to avoid contact with air and impurities. It should not be directly exposed to sunlight to prevent its degradation and deactivation.

Tris plays an important role in biochemistry and biomedical research. Tris analytical purity has high purity and stability, and by fully understanding it, it can be better utilized for experimental research and analysis. As an advantageous supplier of Tris, Desheng produces buffering agents of analytical grade with stable performance and strong buffering ability, which can meet various experimental needs of customers. If you are interested, please click on the website to inquire and purchase!