Use Of Biological Buffer Trihydroxymethyl Aminomethane CAS 77-86-1

Trihydroxymethyl aminomethane, English name: Tris base, CAS No. 77-86-1, white crystalline powder in appearance, soluble in ethanol and water, but also soluble with ethyl acetate, but relatively slow, carbon tetrachloride, corrosive to copper and aluminum. As a common buffer and reagent, it has been widely used in the fields of chemistry, biology and molecular biology, and because of its excellent buffer performance and versatility, it has become one of the indispensable reagents in the laboratory. What aspects can it be applied to? 

Tris parameters:

1, Use of buffer

Tris, as a weak base, can be used to prepare buffer solutions to help maintain the acid-base balance of the solution. Its buffer range is usually between pH 7 and 9, making it suitable for many biological and chemical experiments. In addition, it can also be used in enzyme reaction, Protein purification, DNA/RNA extraction, electrophoresis and other experiments to provide stable environmental conditions.

2, DNA/RNA electrophoresis

Tris EDTA (TE) buffer is one of the commonly used DNA/RNA electrophoresis buffers, where Tris is used to maintain the alkalinity of the solution and EDTA is used to chelate metal ions to prevent enzyme activity. It can also be used for preparing DNA samples, nucleic acid electrophoresis separation and analysis, helping to maintain the stability and visual analysis of DNA/RNA.

3, Protein Research

Tris buffer can be used for protein extraction, purification, and analysis to help maintain protein stability and activity. In Gel electrophoresis of proteins, Tris Glycine buffer system is commonly used in SDS-PAGE to achieve protein separation and quantification.

Tris powder

4, Cell culture

Tris, as one of the components of cell culture medium, can be used to maintain the pH value of the culture medium, provide stable environmental conditions, and dilute and wash cells during cell culture and cell thawing processes to maintain cell health and growth.

5, Biochemical reactions

Tris, as a buffer, plays a crucial role in many biochemical reactions, such as enzyme activity determination, affinity chromatography, and DNA synthesis. It can dilute, store, and stabilize enzymes to maintain their activity and function.

Tris, as a multifunctional chemical reagent, has a wide range of applications. It plays an important role in buffering agents, DNA/RNA electrophoresis, protein research, cell culture, and biochemical reactions. Choosing an appropriate Tris buffer concentration and pH range can provide stable conditions for the experiment and ensure the accuracy and repeatability of the experimental results.

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