High Purity Tris (Hydroxymethyl) Aminomethane Powder Biological Buffer CAS 77-86-1

Tris base is a commonly used buffer. It has good buffer capacity and stability, and can maintain the pH value of solution within a certain range. It is widely used in biochemistry, molecular biology, cell biology, industrial production and other fields. What are the characteristics and uses of Tris base buffer, which was originally produced as white crystalline powder? Below, we will focus on introducing it to everyone.

Tris parameters:

Characteristics of Tris powder

1. Chemical property: Tris powder has high stability at room temperature. Because its solution is weakly alkaline, when the ambient temperature is lower than 25 ℃, its Pka is 8.1. According to the buffer range, its effective buffer PH value is between 7.0 and 9.2.

2. Solubility: Tris powder can be dissolved in water, ethanol and ethyl acetate. It is generally dissolved in deionized water because it is relatively slow to dissolve with ethyl acetate and has corrosive effect on copper and aluminum. At the same time, note that its solubility will increase with the increase of temperature, so it is necessary to adjust the concentration and pH value of the solution according to requirements in the experiment.

3. Buffering capacity: Prepared buffering agents can provide stable buffering capacity within different pH ranges, typically having the best buffering effect between pH 7-9. They have been used in many biological experiments to maintain pH stability in organisms.

tris powder

Use of Tris

1. Gel electrophoresis of proteins: Tris buffer is widely used in Gel electrophoresis of proteins experiments to maintain the pH stability of gel and electrode solution in gel electrophoresis system, so as to ensure the stable operation of gel electrophoresis.

2. DNA/RNA gel electrophoresis: it is used in DNA/RNA gel electrophoresis experiments to prepare agarose gel and electrode buffer to ensure the stability of DNA/RNA migration in electric field.

3. Cell culture: Tris buffer can be used in buffer solutions for cell culture to help maintain the stability of the cell culture environment and promote cell growth and proliferation.

4. Pharmaceutical intermediates: In the process of Drug development, Tris can be used as a pharmaceutical intermediate to help adjust the pH value and stability of drugs.

5. Biochemical experiments: Used as a buffer for reaction systems in many biochemical experiments to help maintain the stability and efficiency of reactions.

Tris is a commonly used buffer, which has good chemical properties and solubility. At the same time, because of its stable buffer capacity and versatility, it plays an important role in scientific research and experimental applications. As an advantageous manufacturer of buffering agents, Desheng has a purity of up to 99% and is commonly used in various experimental studies to achieve stability. It also offers discounted factory prices. If you are interested, please feel free to contact us for purchase!