The PH Range Of High Purity White Powder Good'S Buffer Tris

Tris buffer is widely used in biochemistry, molecular biology and biotechnology experiments. Its appearance is white powder, CAS 77-86-1, with a purity of up to 99%. It has good buffering performance and can adjust the pH value of the solution to maintain stability within a specific pH range. Below is a detailed introduction to help everyone better understand its application scope.

Tris buffer parameters:

PH range of Tris buffer

The pH range of Tris buffer is usually between 7.2 and 9.0. Within this range, Tris buffer exhibits good buffering performance and can effectively maintain the pH stability of the solution. Because in the laboratory, Tris buffers with different pH values are often selected as needed to adjust the acidity and alkalinity of the experimental solution.

Buffering mechanism

The Tris buffering mechanism is mainly achieved through the reaction of amino and hydroxyl groups in Tris molecules with H+ions. Under alkaline conditions, Tris molecules receive H+ions to form Tris H+, which increases the pH of the solution; Under acidic conditions, Tris molecules release H+ions to form Tris H, which reduces the pH of the solution. Therefore, this buffering mechanism enables Tris buffers to have excellent buffering performance over a wide pH range.

tris powder

Application scope

Due to the wide pH range of Tris buffer, it has been widely used in many biochemical and molecular biology experiments. For example, in nucleic acid electrophoresis and Gel electrophoresis of proteins, Tris buffer is often used to adjust the pH value of gel so that DNA or protein can migrate under specific pH conditions. In addition, it is also commonly used in experiments such as cell culture and Protein purification to adjust the pH value of culture medium and washing buffer to ensure the stability of experimental conditions.

When using Tris buffer, the following points should be noted:

1. Tris buffer is sensitive to oxygen and is prone to pH changes due to the influence of oxygen in the air. Therefore, after preparing the solution, attention should be paid to timely sealing and not staying for a long time.

2. Tris buffer is prone to reactions under high temperature conditions, which affects the accuracy of pH value. Therefore, special care should be taken when using it in high-temperature environments.

3. Due to the inherent alkalinity of Tris buffer, it is not suitable for experiments with low pH requirements.

Tris buffer is a commonly used biochemical buffer, with a pH range of 7.2 to 9.0 and excellent buffering performance. In biochemical and molecular biology experiments, Tris buffer is widely used to regulate the pH value of experimental solutions to ensure the accuracy and stability of the experiment. The correct and reasonable selection and use of buffering agents can better play their role. As a professional manufacturer of biological buffering agents, Desheng's products are of analytical purity, with good water solubility, easy preparation, and simple use. If you have any related needs, please feel free to contact us for purchase!