TRIS Base CAS# 77-86-1 Biological Buffers Ph 7-9.2 Buffer Preparation Tris Buffer

TRIS base CAS# 77-86-1 biological buffers ph 7-9.2 Buffer Preparation Tris buffer

Advantages of Biological Buffer Tris

TRIS Chinese name: Tris (hydroxymethyl) aminomethane, white crystalline powder in appearance, good water solubility, can also be dissolved with ethyl acetate but the dissolution rate is relatively slow,carbon tetrachloride, copper, aluminum There is a corrosive effect. Tris has a PKA value of 8 at 25°C and a buffer range of 7-9. Common Tris buffers include Tris-HCL buffer, Tris-phosphate buffer and a variety of derivative buffers, such as TBS, TE, etc. TRIS is a very important biological buffer, the CAS number is 77-86-1.

In biochemical research, biological buffers are often used, and there are as many as 20-30 varieties of biological buffers. Biological buffers are widely used in the in vitro diagnostic industry and biochemical experiments. What are the advantages of Tris in biological buffers?

1. Tris has high solubility in water and is inert to the reaction of many enzymes.

2. Tris has a high buffering capacity. It is generally used to stabilize the pH of the reaction system. It has a strong buffering capacity between pH 7.5-9.0.

3. Tris is very alkaline, so this buffer system can be used to configure a wide range of pH buffers ranging from acidic to alkaline.

4. Tris has little interference to the biochemical process and will not react with calcium, magnesium ions and heavy metal ions.

5. Tris buffer is widely used, and plays an important role in the fields of biochemistry, cosmetics, in vitro diagnostics, molecular biology and coatings.

When using Tris buffer, it is also necessary to note that the pH value of Tris is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process. Tris will easily absorb CO2 in the air, and the prepared buffer should be tightly sealed to avoid loss. In addition, Tris will react with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals. It can also act with heavy metals, but it also has an inhibitory effect on some systems.

Tris buffer pH range

Tris buffer is a widely used buffer in biochemical research. Its commonly used effective pH range is in the "neutral" range, such as:

Tris-HCl buffer: pH=7.5～8.5

Tris-phosphate buffer: pH=5.0～9.0

In addition to TRIS-hcl, TRIS also has a variety of derivatization buffers:

TBS=Tris-HCl+NaCl+KCl is often used to clean the Western blotting membrane in immunostained tissue or Western blotting;

TBST=Tris-HCl+NaCl+tween20, a membrane buffer commonly used in Western Blotting;

TE=Tris-HCl+EDTA, has a protective effect on DNA bases, and is often used for DNA stabilization and storage;

TAE = Tris base + acetic acid + EDTA, is a buffer system widely used in short DNA electrophoresis;

TBE = three bases + boric acid + EDTA, suitable for long-term DNA electrophoresis, and has a good separation effect on small fragments.

Configuration of Tris buffer solution

Advantages of Tris buffer

① Because the Tris base is highly alkaline, this buffer system can be used to prepare buffers with a wide range of pH values ​​ranging from acidic to alkaline;

② It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions.

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Disadvantages of Tris buffer

① The pH of the buffer is greatly affected by the concentration of the solution, the buffer is diluted ten times, and the pH change is greater than 0.1;

② The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution. △pKa/℃＝-0.031, for example: the pH of the buffer solution at 4℃=8.4, then the pH=7.4 at 37℃, so it must be Prepare at the temperature of use. Tris-HCl buffer prepared at room temperature cannot be used at 0℃～4℃;

③ It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;

④ This buffer has a certain interference effect on some pH electrodes, so use an electrode compatible with Tris solution.