The Principle and Preparation Method of Electrophoretic Buffer Trihydroxymethylaminomethane

Electrophoresis is a commonly used technique in biological and chemical experiments, and trimethylaminomethane is a commonly used buffer in electrophoresis experiments. Understanding the principle and preparation method of trimethylolaminomethane is of great significance for conducting electrophoresis experiments. Therefore, this article will provide a detailed introduction to the principle, function, preparation method, and precautions of trimethylolaminomethane.

1, Working principle

Trihydroxymethylaminomethane is a weak base with the molecular formula C4H9NO3. In electrophoresis experiments, trimethylaminomethane mainly plays a role in regulating the pH of the solution. The principle is that trimethylaminomethane molecules contain amino and carboxyl groups, which can dissociate H+and OH - in water, thereby regulating the acidity and alkalinity of the solution. In addition, trimethylaminomethane also has stable DNA and protein properties, and an appropriate pH value protects DNA and proteins from acid-base damage.

2, Function

Adjusting pH: Trihydroxymethylaminomethane can neutralize H+or OH - in the solution, thereby adjusting and stabilizing the pH value easily. In electrophoresis experiments, an appropriate pH can ensure the stability of DNA and proteins, and improve the separation efficiency of electrophoresis.

Protecting DNA and proteins: Trihydroxymethylaminomethane can bind to DNA and proteins, reducing their damage in an electric field and improving the resolution of electrophoresis separation.

Improving conductivity: The amino and carboxyl groups in the trimethylaminomethane molecule can dissociate H+and OH -, improving the conductivity of the solution. In electrophoresis experiments, high conductivity can accelerate the conduction speed of current and shorten electrophoresis time.

trimethylaminomethane powder

3, Preparation method

Mother liquor preparation: Dissolve trimethylaminomethane powder in distilled water to produce 1M trimethylaminomethane mother liquor. Be careful to slowly add trimethylaminomethane powder and stir while adding to avoid clumping.

Preparation of working solution: According to experimental needs, add an appropriate amount of mother liquor to distilled water or buffer to produce a suitable concentration of trimethylaminomethane working solution. In general, the concentration of trimethylaminomethane is between 0.05M and 0.2M.

4, Precautions

Trihydroxymethylaminomethane should be stored in a sealed container to avoid moisture absorption. Before use, check for impurities or particulate matter in trimethylaminomethane, and filter it if necessary.

When preparing working fluids, glass or plastic containers should be used. Metal containers may react chemically with trimethylaminomethane, which can affect the experimental results. Therefore, metal containers should be avoided as much as possible.

Trihydroxymethylaminomethane solution is recommended to be prepared and used immediately. If the solution cannot be used up, it should be stored at 4 ℃ for a short period of time and -20 ℃ for a long period of time.

During the preparation and use process, protective measures should be taken to avoid direct contact with human skin. If accidentally touching the skin, there is no need to panic. First, rinse with plenty of clean water. If you still feel uncomfortable, seek medical attention as soon as possible.

When conducting electrophoresis experiments, an appropriate concentration of trimethylaminomethane buffer should be selected according to the experimental requirements.

Product packaging

Trihydroxymethylaminomethane plays an important role as an electrophoretic buffer in biological and chemical experiments. Therefore, selecting high-quality Trihydroxymethylaminomethane can avoid errors in electrophoretic experimental results. The bio buffering agents produced by Desheng, such as trimethylaminomethane, have high purity, low impurities, good water solubility, stable production process, and small batch differences. We have established cooperation with many domestic and foreign enterprises. If you are also interested in such products, please click on the official website to learn more details!