Storage requirements for HEPES buffer CAS 7365-45-9

HEPES is a buffering agent with relatively stable chemical properties and a wide pH buffering range (7.2-8.2). It can provide a constant pH environment to avoid the impact of pH fluctuations on experimental results. In addition, HEPES buffer also has good cell compatibility and is widely used in cell culture experiments. However, in order to maintain its excellent chemical properties and stability, the correct storage method is crucial.

HEPES buffer related parameters

Storage requirements for HEPES buffer

1. Sealed storage: HEPES buffer should be sealed to prevent the entry of moisture and impurities. Ensure that the reagent bottle or container has good sealing performance to prevent moisture, carbon dioxide, and other impurities in the air from affecting its purity and stability.

2. Dry environment: Store HEPES buffer in a dry environment to avoid moisture absorption. Excessive humidity can lead to a decrease in the purity of the buffer, which in turn affects the experimental results. Therefore, a dry environment is more suitable for storing HEPES buffer.

3. Low temperature storage: High temperatures may cause changes in the chemical properties of HEPES buffers. To maintain its stability, the buffer should be stored in a low-temperature environment. The reagent bottle can be placed in a refrigerator or cold water bath, maintaining a temperature of around 4 ° C.

4. Avoid light: Avoid direct sunlight on the HEPES buffer. Long term exposure to light may cause reagent decomposition, affecting its effectiveness. Therefore, when storing HEPES buffer, choose a dark bottle.

5. Avoid storage with metal ions: Metal ions may react with HEPES buffer, affecting their chemical properties. When preparing and using buffering agents, glassware or plastic products should be used to avoid direct contact with metals.

6. Use original packaging: Use original packaging as much as possible to store HEPES buffer. This can ensure that the reagent is in its original state and prevent external factors from affecting it.

hepes powder

Suggestions and precautions for using HEPES buffer

1. Carefully read the reagent manual: Before using HEPES buffer, please read the manual carefully to understand the correct storage and usage methods.

2. Confirm reagent status: Before using HEPES buffer, please confirm whether its color, clarity, pH value, and other indicators are normal. If there is any abnormality, it should be immediately stopped and repurchased.

3. Pay attention to personal protection: When handling HEPES buffer, please pay attention to personal protective measures. Wear protective equipment such as laboratory uniforms, gloves, and goggles to avoid potential injuries.

4. Reasonable planning of procurement plan: Based on your actual needs and experimental plan, plan the procurement plan reasonably. Ensure that the reagents are used up within their validity period or that new reagents are purchased to ensure the smooth progress of the experiment.

5. Regular inspection: Even if stored according to the above requirements, the HEPES buffer may change over time. Therefore, it is recommended to regularly check its color, clarity, pH value and other indicators to ensure its quality and stability. If the above indicators change, it is recommended to stop using them.

6. Attention to expiration date: HEPES buffer has a certain expiration date, and once it exceeds the expiration date, its quality and stability may be affected. Before use, please ensure to understand the expiration date of the reagent and use it according to regulations.

By understanding and following the correct storage methods, users can ensure the quality and stability of HEPES buffer, thereby ensuring the accuracy of the experiment. If you have any doubts about the use and storage methods of HEPES buffer, you can consult Hubei Xindesheng Material Technology Co., Ltd. Desheng is a professional manufacturer of biological buffering agents, with professional technical personnel on standby to answer questions and provide comprehensive technical guidance for users, ensuring efficient use of each product!

FAQ

Is HEPES an acid or base?

HEPES, Free Acid, Molecular Biology Grade, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range.

What is the action of HEPES buffer?

HEPES is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when a cell culture requires extended periods of manipulation outside of a CO2 incubator.

What are the disadvantages of HEPES buffer?

Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.

How long is HEPES stable?

HEPES sodium salt is stable at least three years if stored sealed and kept dry at room temperature. Although Sigma does not assign expiration dates, sodium HEPES should be re-evaluated for continued suitability in user application every three to five years.

Is HEPES soluble in water?

However, the water solubility value of each “Good's” or biological buffer varies considerably, being HEPES and Tris the most water soluble ones.