The wonderful combination of Tris and other chemicals: synthesis and application of new buffer solutions

Tris is a commonly used buffer component widely used in biochemical and molecular biology experiments. It can be used to synthesize new buffer solutions with various chemical substances to meet different experimental needs. This article will provide a detailed introduction to which chemicals Tris base can be used to synthesize new buffer solutions, and explain their applications.

1, Tris hcl is prepared by blending Tris with hydrochloride salts

Tris HCl buffer is a commonly used pH buffer, which is prepared by Tris and HCl according to experimental requirements. The pH value of this buffer can be controlled by adjusting the concentration of Tris and HCl, which is suitable for maintaining the pH stability of the experimental system. It is commonly used in related experimental processes such as protein extraction, separation, and purification.

tris base powder

2. Tris and glycine are prepared into Tricine buffer solution

Tris glycine buffer is prepared by mixing Tris and glycine in a certain proportion. The pH value of this buffer can be controlled by adjusting the concentration of Tris and glycine, and glycine can also provide a certain ion strength. Commonly used to maintain the stability and activity of proteins in biochemical experiments.

3, Tris is prepared with ethylenediaminetetraacetic acid to form Tris acetic acid buffer solution

Tris acetic acid buffer can be obtained by mixing Tris and acetic acid in a certain proportion. The pH value can be controlled by adjusting the concentration of Tris and acetic acid. In addition to being commonly used to maintain protein stability and activity, it can also be used as a buffer for some enzymatic reactions.

4, Tris is mixed with phosphoric acid to form Tris acetic acid buffer solution

Tris phosphate buffer is a mixture of Tris and phosphate in a certain proportion. Tris phosphate buffer is commonly used to maintain protein stability and activity, and is commonly used in biochemical experiments such as polymerase chain reaction (PCR) and electrophoresis.

5, Other synthesis methods

In addition to the common synthesis methods mentioned above, Tris can also be used to synthesize new buffer solutions with other chemicals. For example, Tris can synthesize EDTA Tris buffer with EDTA, which is commonly used as a buffer for some metal ion chelation reactions; Tris can also synthesize boric acid Tris buffer with boric acid, which is commonly used to maintain the pH stability of the experimental system while providing a certain concentration of boron ions. In addition, there are some special synthesis methods, such as using special chemical substances to synthesize buffer solutions with specific functions with Tris.

Product packaging

6, Application scope

Tris can synthesize new buffer solutions with various chemical substances, which are widely used in fields such as biochemistry, molecular biology, and biotechnology. For example, in the process of protein extraction, separation, and purification, it is necessary to use some buffer solutions with stability and activity; In the enzymatic reaction process, it is necessary to use some buffer solutions with appropriate pH and ion strength; In electrophoresis experiments, it is necessary to use some buffer solutions with stable electrophoresis effects and clear background. In addition, some special synthetic liquids can also be applied in specific experiments, such as using EDTA Tris buffer for metal ion chelation reactions.

Tris and other biological buffering agents developed and produced by Hubei Xindesheng Material Technology Co., Ltd. have advantages such as good water solubility, high purity, and strong buffering ability. If you need derivative buffering agents of Tris, Desheng also has professional R&D personnel to provide you with professional technical support. If you are interested, please feel free to contact us