Introduction to Basic Information of Biological Buffer BES CAS 10191-18-1

BES is a white crystalline powder, also known as N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid. It is easily soluble in water and its aqueous solution has good pH buffering performance. Its molecular formula is C6H15NO5S, with a molecular weight of 213.25. Due to its low toxicity and good biocompatibility, BES buffer is highly favored in biological experiments.

The main uses of BES

1. Biological experiments: BES is one of the commonly used buffering agents in biological experiments, especially in environments where pH stability needs to be maintained. For example, when studying the structure and function of DNA, RNA, and proteins, BES can be used to stabilize pH values and ensure the accuracy of experimental results.

2. Cell culture: During the cell culture process, BES can provide a stable growth environment, which helps with cell growth and reproduction. It helps maintain the homeostasis of the intracellular environment and reduces the adverse effects of pH changes on cells.

3. Protein separation and purification: BES plays an important role in the process of protein separation and purification. It can effectively dissolve various biomolecules and salts, help stabilize the structure and properties of proteins, and improve protein recovery and purity.

4. Enzyme kinetics research: BES can be used as a buffer for enzyme kinetics research. Its stable pH environment helps to accurately measure enzyme activity and provides favorable conditions for studying enzymatic reactions.

BES powder

Advantages of BES

1. Stable pH range: BES has a wide pH range and can maintain stability between pH 6.2 and 8.0, providing reliable buffering for various biological experiments.

2. Low toxicity: Compared to other buffering agents, BES has lower toxicity to biomolecules, less impact on cells and enzymes, and reduces interference with experimental results.

3. High solubility: BES has high solubility and can effectively dissolve various biomolecules and salts, which is beneficial for experimental operations and solution stability.

4. Chemical stability: BES has stable chemical properties and is not easily reactive with other reagents, reducing errors caused by chemical reactions in experiments.

Notes on using BES

1. Concentration control: When using BES as a buffer, its concentration should be strictly controlled to ensure stable pH value of the solution. Meanwhile, excessive concentrations may have adverse effects on certain biomolecules.

2. Avoid high-temperature treatment: BES may decompose or deteriorate at high temperatures, affecting its buffering performance. Therefore, high temperature environments should be avoided during storage and use.

3. Short term storage: BES should be stored in a sealed and dark environment for a short period of time to avoid long-term exposure to air and deterioration.

4. Avoid contact with strong oxidants: BES should avoid mixing with chemical substances such as strong oxidants to prevent chemical reactions from affecting its performance.

5. The use of BES in cell culture: When using BES in cell culture, it should be ensured that its concentration and pH value are appropriate to maintain normal cell growth and metabolism. At the same time, regularly replace the culture medium to ensure the healthy state of the cells.

6. Storage conditions: To maintain the performance and stability of BES, it should be stored in a sealed, dry, cool place, and avoid direct exposure to sunlight or high temperature environments. For long-term storage, it is recommended to regularly check its quality and expiration date.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the production of biological buffering agents such as BES. It has advanced equipment and excellent technical talents, and a factory of over 3000 square meters ensures supply capacity. A professional laboratory is the foundation of product research and development, and nearly 20 years of research and development experience can meet the different needs of users! If you have a recent need to purchase biological buffering agents, please click on the Desheng official website to learn more details!

FAQ

What is the use of BES buffer?

BES buffer is suitable for use in systems where the formation of a calcium phosphate-DNA complex is desirable (Absorbance: < 0.05 at 260 nm, 100 mM). Further, BES buffer can be employed in the study of molecular biology, biological buffers, molecular biology reagents, and zwitterionic compounds.

What is the full form of BES buffer?

BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer.