Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: 68399-80-4
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 20kg / box
Delivery Time: 5 days
Payment Terms: L/C, D/P, T/T, Western Union
Supply Ability: 5t/week
Appearance: |
White Powder |
Model: |
DIPSO Buffer |
Manufacturer: |
Desheng |
Packing: |
500g / Box |
Appearance: |
White Powder |
Model: |
DIPSO Buffer |
Manufacturer: |
Desheng |
Packing: |
500g / Box |
In biological experiments, the use of buffering agents is crucial as they play a crucial role in maintaining the stability of the experimental environment and ensuring the accuracy of experimental results. Among numerous buffering agents, DIPSO buffer has become the best choice for many biological experiments due to its unique chemical properties and good buffering performance. However, just like any chemical reagent, the use of DIPSO also requires attention to some key details and considerations.
Chemical Name | 3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxy-1-propanesulfonic acid |
CAS NO | 68399-80-4 |
Molecular formula | C7H17NO6S |
Molecular weight | 243.28 |
Melting point | 189-192 °C(lit.) |
Density | 1.494±0.06 g/cm3(Predicted) |
Storage conditions | Sealed storage at room temperature |
1, Characteristics and selection of DIPSO buffer
DIPSO is an organic compound with good water solubility and is a good ionic buffer. Due to its stable chemical properties and good buffering performance, DIPSO is widely used in various biological experiments, such as protein crystallization, enzyme kinetics research, and cell culture. When selecting DIPSO buffer, ensure that its purity meets experimental requirements and avoid using expired or contaminated products.
DIPSO powder
2, Storage and Handling
When storing DIPSO, it should be stored in a dry and cool place, avoiding direct sunlight exposure. Due to its hygroscopicity, DIPSO should be sealed and stored in a dryer or polyethylene bag. When handling DIPSO, chemical protective goggles, lab clothes, and chemical protective gloves should be worn to prevent accidental contact and splashing onto the eyes or skin. If accidentally splashed onto the eyes or skin, rinse immediately with plenty of water and seek medical attention promptly.
3, Precautions during use
When using DIPSO buffer, attention should be paid to the following points: first, the appropriate concentration should be prepared according to the experimental requirements. Excessive concentration may lead to non-specific binding and affect experimental results; A low concentration may not provide sufficient buffering capacity. Secondly, contact between DIPSO and strong oxidants or reducing agents should be avoided, as they may cause DIPSO to decompose or produce toxic byproducts.
During use, always pay attention to safety issues and follow the laboratory's safety regulations. For example, containers for storing and handling DIPSO should be clearly labeled and stored in a safe place. In addition, as DIPSO is a strong acid and strong alkaline buffering agent, special attention should be paid to avoiding contact with the skin and eyes during use. If such a situation occurs accidentally, immediately rinse with plenty of water and seek medical attention promptly.
4, Compatibility with other reagents
In biological experiments, it is often necessary to mix DIPSO with other reagents. However, DIPSO is not compatible with all reagents. Therefore, before use, it is necessary to understand the compatibility of DIPSO with other reagents. If unsure if mixed use is possible, it is recommended to conduct small-scale pilot experiments or consult relevant literature first.
5, Disposal of waste
After using DIPSO buffer, a certain amount of waste will be generated. For these wastes, they need to be properly disposed of according to the laboratory's waste disposal regulations. Generally speaking, discarded DIPSO solutions and other chemicals should be classified and stored, and handed over to a professional waste disposal company for disposal within the specified period in the laboratory. Avoid dumping or mixing different types of waste at will to prevent environmental pollution or safety accidents.
Product packaging
Understanding the precautions for using the biological buffer DIPSO can help achieve more efficient use, while avoiding waste caused by improper operation while accurately obtaining experimental results. If you purchase biological buffer from Desheng, professional technicians will guide you in the correct preparation and use of the product. If you are interested, click on the official website to learn more details!
FAQ
What is the full form of Dipso buffer?
DIPSO, also known as 3-(N,N-Bis[2-hydroxyethyl]amino)-2-hydroxypropanesulfonic acid, is a zwitterionic buffer often referred to as a Goods buffer. Combining with MOPS and HEPES, DIPSO can increase the range of its buffering capacity.