Basic information on biological buffer N - (2-acetylamino) -2-aminoethanesulfonic acid (ACES)

(2-Acetylamino) -2-aminoethanesulfonic acid (ACES) is an organic compound with the molecular formula C4H8N2O4S. It is a white to light yellow powder that is soluble in water and not easily deliquescent, but has hygroscopicity. ACES buffer has a pKa value of 6.3, making it a commonly used acidic buffer. It is worth noting that ACES is sensitive to light and should be stored in a dark place.

The properties of ACES

1. Acid-base properties: ACES is an organic acid with a specific pKa value, which can provide hydrogen ions within a certain pH range to maintain the acidity of the solution.

2. Buffering performance: Due to the pKa value of ACES being close to the physiological pH value (7.4), it can effectively buffer the pH value under physiological conditions. This means that using ACES as a buffer during the experiment can ensure that the pH value in the experimental environment remains stable.

3. Stability: ACES is stable at room temperature, but may decompose under high temperature or acidic conditions. Therefore, it is necessary to pay attention to controlling the temperature and pH value during use.

4. Solubility: ACES is easily soluble in water and can also dissolve in some organic solvents at high concentrations. This makes ACES widely applicable in various biochemical experiments.

ACES Buffer

Preparation method of ACES

There are various methods for preparing ACES, and the following is one of the commonly used preparation methods:

Preparation of raw materials: Acetic anhydride, 2-aminoethanesulfonic acid, water, and sodium hydroxide are required.

Synthesis steps: Dissolve 2-aminoethanesulfonic acid in acetic anhydride, then add sodium hydroxide aqueous solution and stir evenly. Heat the mixture to reflux state and maintain for a certain period of time. After the reaction is completed, cool to room temperature and precipitate crystals. After filtration and drying, ACES can be obtained.

The application of ACES in the field of biochemistry

Due to its excellent buffering performance and stability, ACES has various applications in the field of biochemistry:

1. Protein separation and purification: In the process of protein separation and purification, ACES can be used as an ideal buffer for ion exchange chromatography, hydrophobic interaction chromatography and gel filtration chromatography.

2. Enzyme kinetics research: When studying the kinetic properties of enzymes, ACES can serve as a reaction medium, providing a stable pH environment, and accurately measuring enzyme activity.

3. Molecular biology experiments: In molecular biology experiments such as PCR, DNA sequencing, and gene cloning, ACES is used as a buffer or additive to improve the stability and accuracy of the experiment.

4. Biosensors: ACES can also be used to construct biosensors, improving their response performance and stability through their stable pH buffering ability.

5. Cell culture: In cell culture experiments, ACES can serve as a buffer for the culture medium, providing a suitable pH environment to promote cell growth and maintain the physiological state of cells.

6. Electrophoresis technology: In electrophoresis technology, ACES can be used to improve the separation efficiency and resolution of electrophoresis, especially for the separation of some pH sensitive proteins and nucleic acids.

7. Metal ion chelation: ACES has good coordination ability and can form stable chelates with some metal ions, which has certain application value in the separation and enrichment of metal ions.

8. Chemical reaction medium: Due to its stable acid-base properties and good solubility, ACES can be used as a medium for some acid-base reactions or synthesis reactions, promoting the progress of reactions and improving the purity of products.

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FAQ

What is the use of aces buffer?

ACES is used as buffering component in cell culture media, in both agarose and polyacrylamide electrophoresis, and in isoelectric focusing of proteins. ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions.