Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 25kg / barrel
Delivery Time: 3 days
Payment Terms: L/C, T/T, D/P, Western Union
Supply Ability: 1t / day
CAS: |
75621-03-3 |
Appearance: |
White Powder |
Packaging: |
25kg / Barrel |
Alias: |
CHAPS Buffer |
CAS: |
75621-03-3 |
Appearance: |
White Powder |
Packaging: |
25kg / Barrel |
Alias: |
CHAPS Buffer |
In the field of life sciences, the role and function of proteins are hot research topics. However, protein extraction and separation is a highly challenging task. At this point, the biological buffer CHAPS (3- [3- (cholamidopropyl) dimethylamino] propanesulfonic acid internal salt) became a powerful assistant to scientists.
| Chemical Name | 3- [3- (cholamidopropyl) dimethylamino] propanesulfonic acid internal salt |
| English abbreviation | CHAPS buffer |
| CAS NO | 75621-03-3 |
| Molecular formula | C32H58N2O7S |
| Molecular weight | 614.88 |
| Melting point | 156-158°C (dec.) |
| Density | 1.01 g/mL at 20 °C |
| Storage conditions | 2-8 ° C sealed and light proof |
| Manufacturer | Hubei Xindesheng Material Technology Co., Ltd |
CHAPS buffer is a non denaturing zwitterionic detergent with unique chemical properties, making it play an important role in biochemical experiments. This buffer can effectively dissolve membrane proteins and interrupt the interactions between proteins. In biological experiments, the extraction and separation of membrane proteins has always been a challenge, and the emergence of CHAPS provides scientists with a new solution.
CHAPS buffer
In addition to dissolving membrane proteins, CHAPS also has various other uses. It is suitable for dissolving proteins in isoelectric focusing and two-dimensional electrophoresis. In isoelectric focusing, proteins need to be separated at specific pH values, and CHAPS can help proteins maintain their natural state, thereby achieving better resolution. In two-dimensional electrophoresis, CHAPS can improve the solubility of proteins, enabling scientists to better separate and detect proteins.
In addition, CHAPS is also commonly used for non denaturing (urea free) isoelectric focusing. Traditional denaturation methods, such as using denaturants such as urea, may disrupt the structure and function of proteins. The use of CHAPS can avoid such damage, allowing scientists to gain a deeper understanding of the natural state of proteins.
It is worth mentioning that CHAPS has also been found to achieve excellent resolution for certain subcellular preparations and plant proteins. This means that it is of great significance for studying subcellular structures and plant biology. By using CHAPS, scientists can more accurately understand the complex structure and function within cells.
In addition to its application in protein research, CHAPS also has the ability to extract RNA and DNA, which means it can be used not only in protein research but also in nucleic acid research. This feature makes CHAPS a very valuable tool in the field of life sciences.
However, despite the many advantages of CHAPS, caution is still needed in its use. Because it is a descaling agent and protein lysis solution, it is necessary to avoid mixing it with other descaling agents or protein lysis solutions to avoid chemical reactions or precipitation. In addition, it is necessary to choose appropriate concentrations and usage methods based on specific experimental requirements.
Product packaging
Overall, CHAPS is an efficient and multifunctional biological buffer that not only helps scientists better extract and isolate proteins, but also can be used for nucleic acid research, providing strong support for life science research. By using CHAPS, scientists can gain a deeper understanding of the structure and function of proteins, laying the foundation for future development in the fields of medicine and biotechnology.
Hubei Xindesheng Material Technology Co., Ltd. is based in the market and has always been persistently researching and developing various biological buffering agents. At present, Desheng's technology for producing CHAPS buffering agents is very mature, providing high-quality products with high purity, good water solubility, and strong buffering ability. If you are also interested in biological buffering products, please click on the official website to learn more details or communicate with us!
FAQ
What is the function of CHAPS in buffer?
The CHAPS buffer is ideal for pull down co-immunoprecipitation (i.e.Co-IP) analysis, since it maintains protein conformation as well as protein complex assembly. Following cell lysis and cellular protein solubilization into CHAPS buffer, assembled proteins can be isolated together using immunoprecipitation technique.
What is CHAPS reagent?
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent for solubilizing membrane proteins and breaking protein-protein interactions. 1,2 Commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis,3-5 especially for non- denaturing (without urea) IEF.
