Basic information of biological buffer piperazine-1,4-diethanesulfonic acid monosodium salt PIPES-NA

Piperazine-1,4-diethanesulfonic acid monosodium salt, with the chemical formula C8H17N2NaO6S2, is an organic compound that appears as a white powder and is easily soluble in water. PIPES sodium salt is a stable buffering agent with a high pKa value, which can provide a stable pH environment and help maintain the activity of biomolecules. It plays a key role in fields such as molecular biology, cell culture, and electrophoresis technology.

The use of PIPES sodium salt

As a biological buffer, PIPES sodium salt plays an important role in multiple fields. Firstly, in molecular biology research, it is used to maintain the structural and functional stability of proteins. Secondly, in cell culture, it can provide a suitable pH environment for cells, which helps with their growth and division. In addition, PIPES sodium salt is widely used in electrophoresis technology as a supporting medium for separating biological molecules such as proteins and nucleic acids.

The advantages of PIPES sodium salts

1. High stability: PIPES sodium salt has a high pKa value and can provide a stable pH environment over a wide pH range. This makes it perform well in various biological experiments and less susceptible to external environmental influences.

2. Good cell compatibility: PIPES sodium salt as a buffer has no toxic effect on cells and can be safely used in cell culture. It can maintain an appropriate pH value, promote cell growth and division.

3. Good separation effect: In electrophoresis technology, PIPES sodium salt has a good separation effect. It can provide a good separation medium to effectively separate biological molecules such as proteins and nucleic acids.

Product packaging

Precautions for using PIPES sodium salts

1. Storage and stability: PIPES sodium salts should be stored in a dry, cool place, away from direct sunlight and high temperature environments. Under appropriate conditions, its stability is good, but its quality still needs to be regularly checked.

2. Dosage and preparation: When using PIPES sodium salt, it should be accurately weighed and prepared at an appropriate concentration according to experimental requirements. Excessive or insufficient may lead to inaccurate experimental results.

3. Avoid cross contamination: During the experiment, the sterility and purity of the reagents should be ensured. Avoid direct contact with buffer powder or liquid reagents by hand to reduce the risk of cross contamination.

4. Cytotoxicity testing: For applications involving cell culture, a cytotoxicity test should be conducted before using PIPES sodium salt. Ensure that the buffer is non-toxic or low toxic to cells to ensure the reliability of experimental results.

5. Personal protection: When using PIPES sodium salt for related experimental operations, it is necessary to pay attention to taking protective measures, such as wearing masks, goggles, protective clothing, gloves, etc., to strictly prevent PIPES sodium salt solution or powder from getting on the skin or inhaling into the body, thereby endangering physical health.

6. Waste treatment: The used PIPES sodium salt waste liquid should be treated according to laboratory waste treatment regulations to ensure no environmental pollution.

Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the production of in vitro diagnostic reagents. Since its establishment in 2003, the company has developed products ranging from single blood collection additives to various and diversified reagents such as biological buffering agents, chemiluminescence reagents, and chromogenic substrates. Desheng has a professional research and development team and quality inspection team, strictly controlling the quality of each batch of products sold while reducing costs, Let every customer buy products with high cost-effectiveness, and we also welcome elites in the same industry to communicate and learn from us!