Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms
Minimum Order Quantity: 25kg
Price: Detailed discussion
Packaging Details: 25kg / barrel
Delivery Time: 5 days
Payment Terms: L/C, T/T, D/P, Western Union
Supply Ability: 2t/ day
Proportion: |
1.045-1.065g/cm3 |
Exterior: |
Transparent, Opaque And Translucent |
Valid Period: |
Two Years |
Save: |
Room Temperature And Light-proof Sealing |
Proportion: |
1.045-1.065g/cm3 |
Exterior: |
Transparent, Opaque And Translucent |
Valid Period: |
Two Years |
Save: |
Room Temperature And Light-proof Sealing |
Serum separation gel, as a commonly used laboratory tool, is widely used for the separation and preservation of blood samples. However, in practical operation, it is sometimes found that serum separation gel causes initial hemolysis in blood samples. Hemolysis not only affects the quality of the sample, but may also interfere with subsequent experimental results. Therefore, understanding the factors that cause hemolysis in serum separation gel is of great significance for improving experimental accuracy and reliability.
| Product name | Serum separation gel |
| Appearance | Transparent, semi transparent, opaque |
| Relative gravity | 1.045-1.065g/cm3 |
| Viscosity | 100000 to 300000 millipascals |
| volatile | ≤1.5% |
| Product characteristics | Physiologically inert, insoluble in water, with excellent thixotropic and isolation properties |
| Manufacturer | Hubei Xindesheng Material Technology Co., Ltd |
The components of serum separation gel
The main component of serum separation gel is polymers, which can form a barrier during centrifugation to separate red blood cells, white blood cells, and plasma in the blood. However, certain polymer components may react with certain components in the blood, causing red blood cell rupture and leading to hemolysis.
serum separation gel
Improper use
1. Centrifuge speed and time: Excessive or prolonged centrifugation speed may cause red blood cells to experience excessive centrifugal force, leading to rupture. Therefore, when using serum separation gel, appropriate centrifugation speed and time should be selected based on the sample type and centrifuge performance.
2. The ratio of blood to serum separation gel: The ratio of blood to serum separation gel is also an important factor affecting hemolysis. If the blood volume is too high or too low, it may cause uneven force on red blood cells during centrifugation, thereby increasing the risk of hemolysis.
3. Operation during the mixing process: Improper mixing of blood and serum separation gel, such as excessive stirring or uneven mixing, may also lead to red blood cell rupture.
Saving conditions
1. Temperature: Both too high and too low temperatures can have adverse effects on red blood cells, leading to hemolysis. Therefore, when storing blood samples containing serum separation gel, an appropriate temperature should be selected to avoid the influence of temperature fluctuations on the samples.
2. Light exposure: Prolonged intense light exposure may cause changes in certain components of the blood, leading to hemolysis. Therefore, when storing samples, prolonged exposure to strong light should be avoided.
3. Microbial contamination: Microbial contamination may cause damage to red blood cells in the blood, leading to hemolysis. Therefore, when storing samples, strict adherence to sterile operating procedures should be followed to avoid microbial contamination.
Summary and suggestions
The factors that cause initial hemolysis in blood samples caused by serum separation gel are multifaceted, including the composition of serum separation gel, improper use, storage conditions, etc. To avoid this phenomenon, we should choose an appropriate centrifugation speed and time, control the ratio of blood to separation gel, pay attention to the operation during the mixing process, and choose appropriate storage conditions. Meanwhile, we should also pay attention to the potential impact of individual differences and reagent quality issues on the experimental results. By comprehensively understanding and mastering these factors, we can better use serum separation gel for the separation and preservation of blood samples, thereby improving the accuracy and reliability of the experiment.
Product packaging
Hubei Xindesheng Material Technology Co., Ltd. has a history of nearly 20 years in researching and developing blood collection additives. Currently, the products on sale include serum separation gel, heparin lithium, heparin sodium, EDTA anticoagulants, coagulants, silicides, etc. It is one of the few manufacturers in China that can provide a complete set of blood collection additives. If you need it, please feel free to place an order!
FAQ
What is the function of the serum separating gel?
After centrifugation, the inert acrylic gel at the bottom of the tube normally occupies the middle position between the cells (clot) and the serum, as its density is intermediate between theirs. The gel then serves as a barrier to diffusion, preventing contamination of the serum with cellular components.
What gel tubes are used for separation of cells and serum?
Gel-barrier Tubes.Gel-barrier (mottled red/gray, gold, or cherry red-top) tubes contain clot activator and gel for separating serum from cells but include no anticoagulant.
