MOPS 1132-61-2 Biological Buffer A Key Role In Creatinine Assay Kit (enzymatic Method)

The importance of creatinine testing

Creatinine is a waste product produced by muscle metabolism, mainly filtered by the kidneys and excreted with urine. Therefore, the concentration of creatinine in serum or urine can directly reflect the filtration function of the kidneys and overall health status. High levels of creatinine often indicate impaired kidney function, which may be a sign of kidney disease, dehydration, or other systemic diseases. On the contrary, low levels of creatinine may be associated with muscle atrophy, malnutrition, or abnormal liver function. Therefore, accurate measurement of creatinine levels is of great significance for guiding treatment and monitoring disease progression.

MOPS粉末

Advantages of Enzymatic Determination

Compared to the traditional picric acid method, enzymatic determination of creatinine has significant advantages. It uses creatine kinase to hydrolyze creatinine into creatine and urea, and then converts creatine into quantifiable products through a series of enzymatic reactions, such as the consumption or generation of NADH (nicotinamide adenine dinucleotide reduction). This method not only has high specificity and can effectively eliminate interference from other substances, but also has a fast reaction speed and is suitable for large-scale automated detection, greatly improving detection efficiency and accuracy.

3-morpholinopropane sulfonic acid: the key role of biological buffering agents

In the enzymatic creatinine assay kit, 3-methylpropanesulfonic acid plays a crucial role as a biological buffer. Its importance is mainly reflected in the following aspects:

1. Maintain pH stability: 3-morpholinopropane sulfonic acid is an effective pH buffer that can maintain solution stability over a wide pH range. This is crucial for enzymatic reactions, as enzyme activity is highly dependent on the pH value of the environment. By precisely controlling the pH of the reaction system, the enzyme catalyzed reaction is ensured to proceed under optimal conditions, thereby improving the accuracy and reliability of the measurement.

2. Reduce interference: In complex biological samples, there are multiple substances that may affect the measurement results. 3-morpholinopropane sulfonic acid, through its unique chemical properties, can effectively reduce these non-specific interferences and ensure the specificity of the measurement results.

3. Enhancing reagent stability: As a buffering agent, 3-methylpropanesulfonic acid can also improve the stability of other components in the reagent kit, extend the shelf life of the reagent, and reduce measurement errors caused by reagent deterioration.

The practical application of 3-methylpropanesulfonic acid in creatinine determination

In practical operation, a buffer containing 3-methylpropanesulfonic acid is added to the creatinine assay kit, mixed with the sample, and the enzymatic reaction is initiated. By monitoring the changes in optical signals (such as absorbance) during the reaction process, the creatinine concentration in the sample can be calculated. This process not only has a high degree of automation, but also produces accurate and reliable results, providing timely and accurate diagnostic information for clinical doctors and patients.

Product packaging