Product Details
Place of Origin: hubei
Brand Name: desheng
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms
Minimum Order Quantity: 20kg
Price: Detailed discussion
Packaging Details: 25kg / barrel
Delivery Time: 3 days
Payment Terms: L/C, T/T, D/P, Western Union
Supply Ability: 1t / day
CAS: |
6976-37-0 |
Appearance: |
Biological Buffer |
Packaging: |
25kg / Barrel |
Alias: |
BIS-TRIS Buffer |
CAS: |
6976-37-0 |
Appearance: |
Biological Buffer |
Packaging: |
25kg / Barrel |
Alias: |
BIS-TRIS Buffer |
In the fields of biological science research and biopharmaceuticals, protein purification is a crucial technology that directly affects the purity, activity, and stability of the final product. Efficient and mild purification methods can not only improve protein yield, but also maximize its biological activity, laying a solid foundation for subsequent biological experiments or drug development. Among numerous buffering agents, 2- [bis (2-hydroxyethyl) amino] -2- (hydroxymethyl) -1,3-propanediol hydrochloride, abbreviated as BIS-TRIS HCl, stands out in the field of protein purification due to its unique physicochemical properties and wide application potential.
Product Name | 2- [Bis (2-hydroxyethyl) amino] -2- (hydroxymethyl) -1,3-propanediol hydrochloride |
Chemical abbreviation | BIS-TRIS HCl buffer |
Product appearance | White crystalline powder |
CAS number | 124763-51-5 |
Product advantages | Purity of over 99% and good water solubility |
Manufacturer | Hubei Xindesheng Material Technology Co., Ltd |
1,BIS-TRIS HCl: Superior in nature and widely adaptable
BIS-TRIS HCl, as a good zwitterionic buffer, is endowed with excellent water solubility and pH buffering ability due to its multiple hydroxyl and amino groups in its molecular structure. Its pH buffer range is wide, usually between 6.0 and 8.0, which precisely covers the stable pH range of most proteins, making BIS-TRIS HCl an ideal choice for various protein purification strategies. In addition, the low ionic strength and low viscosity characteristics of BIS-TRIS HCl help reduce non-specific interactions during the purification process, improving the recovery and purity of the target protein.
BIS-TRIS HCl powder
2, Mild environment, protecting protein activity
Proteins, as complex biomolecules with complex functions in living organisms, are extremely sensitive to subtle changes in the environment. The BIS-TRIS HCl buffer system can provide a relatively mild and low interference purification environment while maintaining the stability of the target protein. This not only helps to reduce the risk of protein denaturation during the purification process, but also effectively protects the natural conformation and biological activity of the protein, ensuring that the final protein product obtained has high functionality and biocompatibility.
3, Optimize separation and improve purification efficiency
In the complex process of protein purification, effectively separating the target protein from impurities is the key to ensuring purity. BIS-TRIS HCl can optimize the separation effect of various chromatography technologies (such as ion exchange chromatography, gel filtration chromatography, affinity chromatography, etc.) by adjusting the ionic strength and pH value of the solution. Especially in ion exchange chromatography, the zwitterionic properties of BIS-TRIS HCl help reduce charge shielding effects, improve the binding specificity between target proteins and chromatography media, and achieve more efficient separation and purification.
4, Strong compatibility, adaptable to various purification platforms
With the rapid development of biotechnology, the technical means of protein purification are becoming increasingly diverse. BIS-TRIS HCl, with its excellent chemical stability and wide compatibility, can be adapted to various purification platforms and instruments, including laboratory scale manual operations to industrial production automation systems.
In summary, BIS-TRIS HCl buffer has shown great potential and value in protein purification due to its superior physicochemical properties, mild purification environment, efficient separation ability, wide compatibility, and green and safe characteristics. With the continuous advancement of biotechnology and the deepening of proteomics research, BIS-TRIS HCl is expected to become a core component for more innovative purification strategies.
Product packaging
Hubei Xindesheng Material Technology Co., Ltd., as a high-quality manufacturer specializing in the research and development and production of biological buffering agents, has rich experience in research and development and production. The production process is stable, with small batch differences, and can ensure that the product has a white crystalline powder appearance and excellent solubility. If you have recently purchased bio buffer agents such as BIS-TRIS HCl, please click on the official website to learn more details or contact me directly!