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Wuhan Desheng Biochemical Technology Co., Ltd
Wuhan Desheng Biochemical Technology Co., Ltd
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Application of CAPSO 73463-39-5 in Protein Sequencing and Identification

Product Details

Place of Origin: hubei

Brand Name: desheng

Certification: ISO9000

Model Number: 73463-39-5

Payment & Shipping Terms

Minimum Order Quantity: 20kg

Price: Detailed discussion

Packaging Details: 20kg / box

Delivery Time: 5 days

Payment Terms: L/C, D/P, T/T, Western Union

Supply Ability: 5t/week

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Specifications
Highlight:

CAPSO buffer

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73463-39-5

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Biological Buffer

Appearance:
White Powder
Model:
Capso Buffer
Manufacturer:
Desheng
Packing:
500g / Box
Appearance:
White Powder
Model:
Capso Buffer
Manufacturer:
Desheng
Packing:
500g / Box
Description
Application of CAPSO 73463-39-5 in Protein Sequencing and Identification

Protein sequencing and identification technology, as the cornerstone for revealing protein identity, structure, and interaction mechanisms, is constantly driving advances in biomedical, drug development, and biotechnology. In this complex and delicate process, biological buffering agents play an indispensable role, among which 3- (cyclohexylamine) -2-hydroxy-1-propanesulfonic acid (CAPSO buffer) has become an indispensable component in protein research due to its unique physicochemical properties.

 

Product name 3- (cyclohexylamine) -2-hydroxy-1-propanesulfonic acid Chemical abbreviation CAPSO buffer
CAS NO 73463-39-5 Product appearance White powder
Molecular weight 237.32 Molecular formula C9H19NO4S
Product advantages Good water solubility, purity above 99%, stable production process
Manufacturer Hubei Xindesheng Material Technology Co., Ltd

 

1, Basic characteristics and advantages of CAPSO

 

CAPSO, as a high-performance biological buffer, has a cleverly designed molecular structure that combines good water solubility and pH buffering ability. CAPSO can maintain a stable pH value of the solution within a wide pH range (usually pH 6-11), which is crucial for maintaining the natural conformation of proteins and preventing their denaturation. In addition, CAPSO's low toxicity, low osmotic pressure characteristics, and compatibility with multiple enzymes make it an ideal choice for biological experiments, especially in protein sequencing and identification experiments that require precise control of experimental conditions.

 

Application of CAPSO 73463-39-5 in Protein Sequencing and Identification 0

CAPSO buffer

 

2, Application of CAPSO in Protein Extraction and Purification

 

The first step in protein sequencing and identification is to efficiently and cleanly obtain the target protein. CAPSO played an important role during this stage. By optimizing the buffer system, CAPSO can not only effectively separate proteins from complex biological samples, but also reduce non-specific binding and improve purification efficiency.

 

3, CAPSO optimized protein electrophoresis separation

 

Protein electrophoresis is an important means of identifying protein molecular weight, purity, and subunit composition. CAPSO is often used as a part of electrophoresis buffer due to its excellent buffering performance and low conductivity. CAPSO can effectively stabilize the electric field during electrophoresis, reduce thermal effects and protein aggregation, thereby improving resolution and clarity, allowing proteins with small molecular weight differences to be clearly distinguished, providing reliable basis for subsequent mass spectrometry analysis or antibody specificity verification.

 

4, The auxiliary role of CAPSO in mass spectrometry analysis

 

Mass spectrometry technology has become the gold standard for protein sequencing and identification. CAPSO helps to completely dissociate and ionize proteins by adjusting the pH of sample solutions to the optimal range, thereby improving the sensitivity and accuracy of mass spectrometry detection. Meanwhile, the low background interference characteristic of CAPSO ensures the clarity and readability of the mass spectrum, facilitating subsequent data analysis and accurate inference of protein sequences.

 

5, CAPSO promotes protein structure and function research

 

The function of proteins is often closely related to their three-dimensional structure. CAPSO, as a stabilizer or additive in structural biology research, especially in X-ray crystallography and cryo EM techniques, can effectively maintain the stable conformation of proteins, prevent phase transitions or degradation during crystallization, and assist scientists in deciphering high-resolution protein structures.

 

Application of CAPSO 73463-39-5 in Protein Sequencing and Identification 1

Product packaging

 

In addition to CAPSO buffer, Desheng also produces other high-quality biological buffer products, such as tris besie, tes free acid, and dozens of other products. These products also have good performance and reliable quality, which can meet the needs of different experiments. At present, Desheng has established cooperation with hundreds of domestic and foreign enterprises, which has been widely recognized by customers. This is also a manifestation of the good competitiveness of Desheng products! If you have any recent purchasing needs, please click on the official website of Desheng to learn more details!

 

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