Stability Analysis of Biological Buffer CAPSO 73463-39-5

In biochemical and molecular biology experiments, the stability of buffering agents directly determines the reliability of experimental data. 3- (cyclohexylamine) -2-hydroxy-1-propanesulfonic acid (CAPSO buffer, CAS 73463-39-5), as a zwitterionic buffer, has shown significant advantages in protein purification, electrophoresis analysis, cell culture, and other fields due to its unique chemical structure and pH buffering properties. This article will comprehensively analyze the stability mechanism and influencing factors of CAPSO from the aspects of chemical structure, environmental conditions, etc.

1, Chemical structure: stability cornerstone and functional groups

The molecular formula of CAPSO is C ₉ H ₁ NO ₄ S, with a molecular weight of 237.32. Its core structure consists of cyclohexylamine groups, sulfonic acid groups, and 2-hydroxypropanesulfonic acid chains. This structure endows it with dual buffering properties: the sulfonic acid group (- SO ∝⁻) provides weak acidity, and the cyclohexylamine group (- NH Cyclohexyl) contributes weak alkalinity. The two work together to form a pH buffering range of 8.9-10.3. Compared to traditional buffering agents, the uniqueness of CAPSO lies in the introduction of its hydroxyl group (- OH), which not only enhances the hydrophilicity of the molecule, but also stabilizes the ion balance in the solution through hydrogen bonding, effectively suppressing pH fluctuations.

CAPSO powder

Experimental data shows that the dissociation constant pKa of CAPSO at 25 ℃ is 9.8, which enables it to exhibit better buffering efficiency in weakly alkaline environments. For example, in nitrocellulose membrane protein imprinting experiments, CAPSO can prevent significant changes in pH and prevent the formation of insoluble precipitates between proteins and metal ions, thereby protecting the integrity and activity of membrane proteins. In addition, the chemical inertness of CAPSO ensures that it does not undergo non-specific binding with biomolecules, further enhancing the accuracy of experimental results.

Environmental conditions: synergistic effect of temperature, pH, and ionic strength

The stability of CAPSO is influenced by multiple environmental factors, among which temperature, pH, and ionic strength are key variables:

1. Temperature control: The melting point of CAPSO is 270-274 ℃, indicating its high stability at room temperature. However, high temperatures may cause changes in molecular structure, leading to a decrease in buffering efficiency. Suggest storing at room temperature.

2. PH range adaptation: The buffering range of CAPSO is 8.9-10.3. Exceeding this range may cause dissociation equilibrium shift, thereby reducing buffering capacity.

3. Ionic strength regulation: The ion strength of CAPSO needs to be controlled within the physiological range (0.1-0.2 M), and excessively high or low ion concentrations may affect its buffering effect.

Stability optimization strategy

To improve the stability of CAPSO, it is necessary to start from three aspects: synthesis process, purity control, and application optimization:

Improvement of synthesis process: Adopting a low-temperature reaction method between sodium bisulfite and cyclohexylamine can avoid side reactions caused by high temperatures.

Purity grading management: Analytical grade CAPSO (purity ≥ 99%) is suitable for biological experiments, while industrial grade CAPSO needs to adjust purity standards according to application scenarios.

Product packaging

In addition to CAPSO buffer, Desheng also produces other high-quality biological buffer products, such as tris besie, tes free acid, and dozens of other products. These products also have good performance and reliable quality, which can meet the needs of different experiments. At present, Desheng has established cooperation with hundreds of domestic and foreign enterprises, which has been widely recognized by customers. This is also a manifestation of the good competitiveness of Desheng products! If you have any recent purchasing needs, please click on the official website of Desheng to learn more details!