Standardize the use of chemiluminescence reagent acridine salt NSP-SA powder 211106-69-3

In the fields of biomedical detection and chemical analysis, acridine salt NSP-SA has become a core reagent for labeling proteins, antibodies, nucleic acids and other biomolecules due to its excellent fluorescence performance and chemical stability. However, its active groups are sensitive to environmental conditions, and improper operation can easily lead to reagent degradation or decreased labeling efficiency. This article systematically explains how to standardize the use of acridine salt NSP-SA powder from four dimensions: storage conditions, access standards, dissolution operations, and safety protection.

Storage conditions: Low temperature, light proof, sealed and moisture-proof

The molecular structure of acridine salt NSP-SA contains sulfonic acid groups and carboxyl groups, and is highly sensitive to temperature, humidity, and light. To ensure its stability, the following storage specifications must be strictly followed:

acridine salt NSP-SA powder

1. Temperature control: It is recommended to place the reagent in a low-temperature environment to avoid repeated freeze-thaw cycles that may damage the molecular structure. The laboratory can use professional equipment and regularly calibrate temperature sensors.

2. Light shielding treatment: Reagents should be stored in dark light shielding containers, such as brown glass bottles or aluminum foil bags, to prevent UV induced photolysis reactions.

3. Sealed and moisture-proof: Multiple layers of composite materials are used for packaging, combined with desiccants to prevent moisture from entering the air. Immediately seal after opening to prevent the reagent from absorbing moisture and clumping.

Usage specifications: pre-processing, light avoidance operation, and precise weighing

The use of acridine salt NSP-SA requires a balance between reagent stability and operational accuracy. The specific steps are as follows:

1. Preprocessing: Remove the reagent from the frozen environment 30 minutes in advance and place it in a drying dish to equilibrate to room temperature to avoid condensation of water vapor caused by temperature differences.

2. Light avoidance operation: Weigh in a dark room or yellow light environment, using an electronic balance with a light shield to reduce the risk of light induced degradation.

3. Accurate weighing: Using the skinless weighing method, place the container directly on the balance to zero, and then add the reagent powder. It is recommended to use an analytical balance with a precision of one hundred thousandth to ensure a weighing error of ≤ 0.1mg.

Dissolving operation: solvent selection and concentration control

The solubility of acridine salt NSP-SA directly affects its labeling efficiency, and the following principles must be strictly followed:

1. Solvent selection: Anhydrous N, N-dimethylformamide (DMF) or dimethyl sulfoxide (DMSO) is preferred to avoid the use of aqueous solvents that may cause reagent hydrolysis.

2. Concentration control: Prepare solutions of different concentrations according to experimental requirements.

3. Dissolution conditions: Stir magnetically at room temperature until completely dissolved, avoiding heating to accelerate the degradation of the reagent.

Safety protection: personal protection and waste disposal

Although acridine salt NSP-SA has low toxicity, improper operation may still pose health risks, and the following protective measures should be taken:

1. Personal protection: Laboratory personnel should wear masks, nitrile gloves, and goggles to avoid inhalation or skin contact with reagent powder. If accidentally touched, rinse immediately with plenty of water and seek medical assistance if necessary.

2. Environmental control: Weigh and dissolve in a fume hood to ensure air circulation. The experimental table should be equipped with anti-seepage pads to prevent reagents from spilling.

3. Waste disposal: After use, the container needs to be cleaned three times with ethanol, and the waste liquid needs to be collected in a dedicated container labeled as "chemiluminescence reagent waste" for professional disposal.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as an advantageous supplier of luminescent reagents, has rich production experience and has a history of 20 years since its establishment in 2005. The acridine series products produced include 7 types, which can be fully selected by customers. At the same time, their purity can reach up to 98%, and their luminescence is sensitive and rapid, which is conducive to various experimental research uses. If you have any relevant intentions, please click on the official website to learn more details or contact me!