Application of trihydroxymethylaminomethane 77-86-1 in carbon dioxide determination kit

In the fields of clinical medicine and biochemical testing, accurate determination of carbon dioxide concentration is crucial for diagnosing metabolic diseases, evaluating respiratory function, and monitoring acid-base balance. As a star molecule in the field of biological buffering agents, Tris base(CAS number 77-86-1) has become a core component in the development of carbon dioxide detection kits due to its unique physicochemical properties, providing a solid guarantee for the accuracy and stability of detection results.

1, Tris's physicochemical properties: laying the foundation for buffer systems

Tris is a white crystalline powder with a molecular formula of C ₄ H ₁ N O3 and a molecular weight of 121.14. Its most significant feature lies in its efficient buffering ability: Tris can maintain the dynamic equilibrium of solution pH through protonation and deprotonation reactions within the pH range of 7.0 to 9.2. This characteristic makes it an ideal choice for carbon dioxide detection kits - when carbon dioxide in the sample reacts chemically with the reagent, the pH of the system may fluctuate, and the buffering effect of Tris can effectively counteract this change, ensuring that enzymatic reactions proceed under optimal pH conditions.

TRIS base powder

In addition, Tris's low ionic strength makes it suitable for ion sensitive detection systems, such as experiments on the formation of intermediate fibers in nematode nuclear fiber layer proteins. In carbon dioxide measurement, this characteristic can reduce non-specific ion interference and improve detection sensitivity. It has high water solubility, is easier to prepare reagents, and is compatible with multiple solvents, providing convenience for the industrial production of reagent kits.

2, The synergistic effect of Tris in enzymatic reactions

Modern carbon dioxide detection kits often use enzymatic rate methods to indirectly calculate carbon dioxide concentration by monitoring changes in coenzyme absorbance. Taking the formate dehydrogenase formaldehyde dehydrogenase coupling system as an example, Tris buffer provides a stable pH environment for enzymatic reactions, ensuring that formate dehydrogenase converts carbon dioxide to formic acid and further oxidizes reduced coenzymes (such as NADH) to oxidized coenzymes through formaldehyde dehydrogenase. During this process, Tris maintains pH stability, avoiding a decrease in enzyme activity due to pH fluctuations, thereby ensuring reaction rate and detection sensitivity.

3, Formula optimization: synergistic effect of Tris and stabilizer

To extend the storage life of the reagent kit and reduce cross contamination, Tris is often used in combination with stabilizers in research and development. In addition, Tris can be compounded with other buffer systems to meet the needs of different detection platforms. Tris works synergistically with components such as sodium azide and phosphoenolpyruvate to achieve rapid quantitative determination of human serum carbon dioxide concentration.

4, Application scenarios and future prospects

The carbon dioxide assay kit containing TRIS buffer components has been widely used in clinical emergency, intensive care, and research laboratories. Its advantages lie in easy operation, accurate results, compatibility with mainstream biochemical analyzers, and no need for complex preprocessing. For example, in the diagnosis of metabolic acidosis, the kit can complete the test within 10 minutes, buying valuable time for clinical decision-making.

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