Biological buffer BES 10191-18-1 for free fatty acid assay kit

Accurate detection of free fatty acids (FFA) is a key indicator for evaluating energy metabolism, insulin resistance, and lipid metabolism disorders in clinical diagnosis, nutritional research, and metabolic disease monitoring. However, FFA has lively chemical properties and is easily affected by environmental pH fluctuations, resulting in unstable detection results. At this point, N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (BES buffer), as a high-performance biological buffer, has become an indispensable "invisible guardian" in free fatty acid assay kits due to its excellent buffering performance and biocompatibility.

1, Chemical Characteristics of BES: A Molecular Balance for Precise pH Control

BES is a zwitterionic buffering agent, endowed with strong pH buffering ability by the amino and sulfonic acid groups in its molecular structure. Within the pH range of 6.4-7.8, BES can effectively resist acid-base interference and maintain the pH stability of the reaction system. This feature is crucial for FFA detection:

The pH sensitivity of FFA: Free fatty acids are prone to saponification reaction under alkaline conditions, resulting in artificially high detection values; Under acidic conditions, insoluble salts may form, reducing detection sensitivity.

BES buffer

The buffering advantage of BES: By precisely adjusting the pH of the reaction system to neutral or weakly alkaline (such as pH 7.4), BES can avoid chemical degradation of FFA while ensuring efficient enzymatic reactions (such as esterase catalysis).

2, The core role of BES in free fatty acid assay kit

Protect enzyme activity and enhance detection sensitivity

Free fatty acid detection often relies on esterase catalyzed reaction between FFA and chromogenic substrates. BES maintains pH stability, prevents enzyme protein denaturation, and ensures maximum enzyme activity. For example, in the copper ion colorimetric method, the BES buffer system can significantly enhance the reaction signal intensity, making it possible to detect low concentrations of FFA.

Compatible with multiple detection methods, expanding application scenarios

BES can provide a stable pH environment, whether it is enzymatic colorimetry, fluorescence, or electrochemical methods. For example, in fluorescence detection based on acyl CoA oxidase, the BES buffer system can maintain the quantum yield of the fluorescent probe, ensuring a linear correlation between the signal and FFA concentration.

3, Biocompatibility of BES: Dual guarantee of safety and efficiency

As a member of the Good's buffer family, BES has low cytotoxicity, high water solubility, and chemical inertness. In kit development, this feature means:

Reduce sample pre-processing steps: BES can be directly compatible with serum, plasma, or tissue homogenates without the need for additional neutralization or dilution, simplifying the operational process.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the production of biological buffering agents such as BES. It has advanced equipment and excellent technical personnel to ensure product quality, provide good customized services and after-sales services. If you have a recent need to purchase biological buffering agents, please click on the official website of Desheng to learn more details!