The impact of high concentration of biological buffer BES 10191-18-1 on experimental results

In the precise world of biological experiments, every detail can become the key to success or failure. The biological buffer N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (BES buffer) plays an important role in maintaining the pH stability of the experimental system, and its concentration control is crucial. Once the concentration of BES is too high, it is like throwing a giant rock into a calm lake, which will have many adverse effects on the experimental results.

Interference with normal physiological functions of cells

Cells are the core research objects of biological experiments, and they are extremely sensitive to subtle changes in the surrounding environment. When the concentration of BES is too high, it can disrupt the delicate state of ion balance and osmotic pressure inside and outside the cell. The cell membrane serves as a barrier between cells and the external environment, and its normal function depends on specific ion concentration gradients. High concentrations of BES may interfere with the normal operation of ion channels and transporters on the cell membrane, leading to abnormal ion transmembrane transport. For example, sodium ions, potassium ions, etc. that should have been orderly entering and leaving the cell can be disrupted by the influence of BES, which in turn affects the cell's resting potential and action potential, interfering with the cell's signal transduction process.

BES powder

Affects protein structure and activity

Proteins are the main carriers of life activities, and many biological experiments revolve around proteins. Excessive concentration of BES can significantly affect the structure and activity of proteins. The three-dimensional structure of proteins is the basis for their functions, and high concentrations of BES may interact with amino acid residues on the surface of proteins, altering their conformation. This conformational change may lead to the masking or destruction of the active center of the protein, causing the protein to lose its original catalytic, binding, and other activities.

Chemical reactions that interfere with experimental systems

In biological experiments, various chemical reactions are often involved, such as nucleic acid amplification and antibody binding. Excessive concentration of BES can alter the ion strength and pH environment of the reaction system, thereby interfering with the progress of these chemical reactions. Taking PCR reaction as an example, appropriate ion strength and pH are key factors in ensuring DNA polymerase activity and primer template binding efficiency. High concentrations of BES may cause excessive ion strength in the reaction system, affecting the stability of DNA polymerase, reducing its amplification efficiency, and even leading to amplification failure. In addition, in immunological experiments, excessive concentration of BES may affect the binding affinity between antibodies and antigens, leading to weakened detection signals or false negative results, which can affect the accuracy of experimental results.

Increase experimental error and uncertainty

Excessive concentration of BES can also increase experimental errors and uncertainties. Due to its multifaceted effects on cells, proteins, and chemical reactions, experimental results may experience significant fluctuations. The impact of high concentrations of BES may vary across different batches or experimental conditions, making it difficult to replicate and validate experimental results. Researchers face greater difficulties in conducting data analysis, as they are unable to accurately determine whether experimental results are true phenomena or interference caused by high BES concentrations. This not only wastes a lot of time and resources, but may also mislead research directions.

Product packaging

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