Analysis of the advantages of high absorption wavelength of chromogenic substrate MAOS 82692-97-5

In the field of biochemical detection, chromogenic substrates are the "bridge" connecting target molecules with observable signals, and their performance directly affects the sensitivity, anti-interference ability, and applicable scenarios of detection. N-Ethyl-N - (2-hydroxy-3-sulfopropyl) -3,5-dimethylaniline sodium salt (MAOS), as the new chromogenic substrate, has shown significant advantages in clinical diagnosis, environmental monitoring, and other fields due to its unique high absorption wavelength characteristics.

1, Scientific principle: Molecular design wisdom with high absorption wavelength

In the molecular structure of MAOS, the 3,5-dimethylaniline group serves as the chromogenic center and works synergistically with the sulfopropyl side chain and ethyl substituent, resulting in a maximum absorption wavelength of 630nm. High wavelength photons have lower energy and better match the energy required for electron transitions in MAOS molecules, thereby reducing energy waste and improving light absorption efficiency. The aniline group forms an extended conjugated system with the sulfopropyl side chain, which increases the electron delocalization range and shifts the absorption spectrum towards longer wavelengths. This precise molecular level regulation enables MAOS to capture light energy more efficiently in color reactions, laying the foundation for subsequent signal amplification.

MAOS powder

2, Anti interference capability: breaking through the bottleneck of complex sample detection

In real detection scenarios, there are often multiple interfering substances (such as hemoglobin, bilirubin, lipids, etc.) in the sample, which have strong absorption in the low wavelength region (such as 400-500 nm) and are prone to masking the target signal. The high absorption wavelength characteristic of MAOS endows it with a unique ability to penetrate interference:

Avoiding endogenous interference: Interference substances in biological samples (such as serum and urine) absorb significantly at wavelengths below 500 nm, while the 630 nm absorption peak of MAOS can completely avoid this region, significantly reducing background noise and improving signal-to-noise ratio.

Reduce the impact of light scattering: High wavelength light scatters weaker in turbid samples (such as water containing particles), and the color signal of MAOS can penetrate the sample more stably, ensuring the reliability of detection results.

For example, in the detection of myocardial injury markers such as troponin, lipid particles in serum often cause fluctuations in the signal of traditional chromogenic substrates, while MAOS's orange red color can penetrate the lipid layer, making the results more stable and reproducible.

3, Application scenario expansion: comprehensive empowerment from laboratory to on-site

The high absorption wavelength advantage of MAOS not only improves detection performance, but also expands its application boundaries, making it an ideal choice for multi scene detection and a "precise tool" for clinical diagnosis. In enzyme-linked immunosorbent assay (ELISA), MAOS combined with peroxidase catalytic system can produce stable color at 600 nm, which is suitable for the detection of low concentration biomolecules such as tumor markers and hormones, and helps with early disease screening.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. has won wide recognition in the market for producing high-quality chromogenic substrates. Currently, in addition to MAOS reagent, other chromogenic substrates available for sale include TOOS, TOPS, ADOS, ADPS, etc. Desheng has a professional technical team to provide customers with excellent after-sales service. If you have any related purchasing needs, please click on the official website to learn more details or contact me!