What is the color rendering effect of TOPS 40567-80-4 in uric acid detection kit?

In today's increasingly refined management of chronic diseases, uric acid testing has become a core indicator for the diagnosis and monitoring of diseases such as hyperuricemia and gout. Traditional uric acid detection methods often face pain points such as insufficient sensitivity and poor anti-interference. However, N-ethyl-N - (3-sulfopropyl) -3-methylaniline sodium salt (TOPS reagent), as a new generation of colorimetric reagent, is redefining the accuracy and reliability of uric acid detection due to its unique chemical properties. This article will analyze how TOPS becomes the "color engine" in the field of uric acid detection from three dimensions: mechanism of action, color advantages, and application scenarios.

The color development principle of TOPS

TOPS is a key chromogenic substrate in the novel Trinder reaction system, and its molecular structure is endowed with dual functions by the sulfopropyl and methylaniline groups

TOPS Powder

1. Specific binding of uric acid oxidase products: In uric acid detection, uric acid is first broken down by uric acid oxidase into uracil and hydrogen peroxide. TOPS, as a substrate for peroxidase (POD), can undergo oxidative condensation reactions with H ₂ ₂ and 4-aminoantipyrine (4-AAP) to generate stable quinone imine dyes (red complex).

2. The "directional navigation" of sulfonic acid groups: The sulfopropyl group (- SO3H) in the molecule enhances its affinity with enzyme reaction products through electrostatic interactions, while improving the water solubility of the reagent, ensuring efficient reaction in a homogeneous system. This feature enables TOPS to maintain high selectivity in complex biological samples such as serum and plasma, avoiding the false triggering of common interferents such as bilirubin and vitamin C.

Four core advantages: redefining uric acid testing standards

1. Sensitivity jump: The quinone imine dye generated by TOPS has a strong absorption peak at a wavelength of 546nm, with a molar absorptivity of up to 2.5 × 10 ⁴ L/(mol · cm), which is improved compared to traditional color reagents. This means that even when the concentration of uric acid in the sample is low, reliable detection can still be achieved through changes in absorbance, providing technical support for early screening of hyperuricemia.

2. Anti interference ability: In clinical samples, substances such as bilirubin and vitamin C often cause color shift. TOPS can effectively inhibit non-specific adsorption through the electrostatic shielding effect of sulfonic acid groups. Combined with an optimized buffer system, the correlation between detection results and true uric acid concentration is improved.

3. Stability breakthrough: The sodium salt form of TOPS has significant chemical stability and can be stored for a long time in a suitable storage environment without affecting its performance or causing deterioration.

4. Compatibility Expansion: Compatible with multiple detection platforms, TOPS can achieve flexible adaptation by adjusting concentration and reaction time, whether it is a fully automatic biochemical analyzer, portable uric acid analyzer, or microfluidic chip laboratory. This strong compatibility feature makes it the preferred color rendering solution for real-time detection device upgrades.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as a high-quality developer and manufacturer of chromogenic substrates, can provide high-quality diagnostic reagent raw materials for in vitro diagnostic kit manufacturers. Desheng has professional experimental equipment and technical personnel, and can provide good customized and after-sales services. If you are also interested in TOPS and other new trinder's reagents, please click on the official website to learn more details or contact me!