Application of TOOS in in vitro diagnostic reagents CAS82692-93-1

In vitro diagnostic (IVD) kits, TOOS reagent(N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt) is an important chromogenic substrate material, and its chromogenic stability plays a key role in the detection results. The color stability of TOOS is reflected in multiple aspects, including its own chemical structure characteristics, performance under different environmental conditions, reaction characteristics with other reagents, and changes during long-term storage.

1, Stability determined by the chemical structure foundation

The sulfopropyl and other functional groups contained in the TOOS molecular structure enhance its water solubility and chemical stability. The presence of sulfopropyl groups enables TOOS to be uniformly dispersed in aqueous solutions, making it less prone to aggregation or precipitation. This lays the foundation for its stable color performance in complex biological sample detection environments. Compared to some structurally simple chromogenic substrates, TOOS's structure allows it to more stably and specifically bind to other reagents during chemical reactions, reducing the occurrence of side reactions.

TOOS Powder

2, Tolerance to changes in environmental factors

In the actual IVD detection process, the reagent kit may experience different temperature environments, from production and storage to transportation and then to the site of use. TOOS exhibits stability in the face of temperature changes. Within a reasonable temperature range, although the thermal motion of TOOS molecules may change, it will not have a substantial impact on their chemical structure and color rendering properties. However, if the temperature is too high, such as exceeding 60 ℃, it may accelerate the thermal motion of its molecules, leading to the breaking of chemical bonds within the molecules and affecting color stability. Overall, TOOS can maintain its color stability well within the common temperature range related to IVD detection.

3, Stability performance during the reaction process

In IVD kits, TOOS typically needs to interact with multiple reagents to complete color reactions, such as oxidative coupling reactions with peroxidase, hydrogen peroxide, and other auxiliary substrates. TOOS exhibits good specificity and stability in these reaction processes. It can accurately and specifically bind with the target reagent, react according to the predetermined chemical reaction pathway, and generate stable color products. In the blood glucose detection kit, TOOS and glucose oxidase catalyze the oxidation of glucose to produce hydrogen peroxide, which reacts with peroxidase to produce stable quinone imine dyes. The color depth is proportional to the blood glucose concentration. This specific reaction ensures that TOOS can accurately indicate the presence and content of target substances in complex biological samples, and the reaction process is stable and not easily disturbed by other components in the sample.

4, Long term storage stability

When not in use, TOOS typically exists in the form of solid powder in IVD kits. Under appropriate storage conditions, such as sealed storage in a cool, dry, and dark environment, TOOS solid powder can remain stable for a long time. In the solid state, the interactions between TOOS molecules are relatively weak, and they have less contact with oxygen, water, and other substances in the external environment, making them less prone to oxidation, hydrolysis, and other reactions. Studies have shown that under the ideal storage conditions mentioned above, TOOS solid powder can still maintain its original chemical activity and color development performance within 1-2 years, providing a longer shelf life for IVD kits and ensuring that the detection performance of the kit is not affected by changes in TOOS during storage.

Product packaging

TOOS, as a chromogenic substrate material for IVD assay kits, exhibits color stability in various aspects such as chemical structural characteristics, tolerance to environmental factors, performance during reaction processes, and long-term storage. In the development, production, storage, and use of IVD kits, fully considering and utilizing the color stability characteristics of TOOS can improve the detection accuracy of the kit. As a research and development manufacturer of TOOS substrate, Hubei Xindesheng has always adhered to technological innovation, relying on a professional R&D team and advanced production equipment to continuously optimize the TOOS synthesis process and improve the purity and performance of TOOS. With a persistent pursuit of quality, New Desheng's TOOS products perform well in color stability, specificity, and other aspects, meeting the application needs of different IVD test kits. If you need TOOS as a chromogenic substrate, please feel free to contact us at any time!