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521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB

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521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB

521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB
521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB

Large Image :  521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB

Product Details:
Place of Origin: HUBEI,CHINA
Brand Name: DESHENG
Certification: ISO9000
Model Number: AR
Payment & Shipping Terms:
Minimum Order Quantity: 500g
Price: Consult the latest quotation
Packaging Details: 500g / bottle, 25kg / barrel
Delivery Time: 5 days
Payment Terms: L/C, D/P, T/T, Western Union
Supply Ability: 100kg/a week
Detailed Product Description
Brand: Desheng Place Of Origin: Hubei China
Purity: >98% Appearance: Yellow Powder
Molecular Formula: C8H7N3O2 Molecular Weight: 177.16
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521-31-3 Luminol Hrp

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WB Luminol Hrp

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CAS 521-31-3 chemiluminescent hrp substrate

Application of chemiluminescent substrate luminol in western blot WB;CAS 521-31-3;Purity 98% ;Yellow powder

 

Application of chemiluminescent substrate luminol in western blot WB


Luminol, also called luminol, can detect a very small amount of hydrogen peroxide. It is usually used as a substrate for peroxidase for various biochemical detections. It is very sensitive and easy to automate. Among them, Western blot WB experiments are often used. Use ECL luminescent liquid made of luminol.

CAS 521-31-3
Another name LUMINOL; 3-Aminophthalhydrazide; 521-31-3; 5-Amino-2,3-Dihydrophthalazine-1,4-Dione; 5-Amino-2,3-Dihydro-1,4-Phthalazinedione
Purpose Used as chemical analysis reagent and indicator
 

 

521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 0

In Western Blot (Western Blot), that is, Western blotting, the ECL luminescent solution used is made of luminol. Luminol luminescence immunoassay is usually the last step of Western Blot, adding 1:1 solution A and solution B, covering the pvdf film, you can observe the experimental results by luminescence. The luminescent liquids A and B here are solutions made of luminol and hydrogen peroxide, respectively.
521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 1
ECL luminous liquid raw material luminol luminol
Western blotting is a protein detection technique in which the total proteins of cells or tissues are separated by electrophoresis and transferred from the electrophoresis gel to a solid support NC membrane or PVDF membrane, and then a specific antibody is used to detect a specific antigen. It is now widely used It is used in many aspects such as gene expression research at the protein level, antibody activity detection and early disease diagnosis. After the target protein is labeled with HRP enzyme, it can be observed and detected by luminol luminescence.
Western Blot is similar to Southern Blot or Northern Blot, but the WB method uses polyacrylamide gel electrophoresis, and the object to be detected or blotted is protein. The protein or polypeptide sample is separated by PAGE and transferred to the carrier nitrocellulose membrane. The solid-phase carrier adsorbs the protein by non-covalent bonds and ensures its activity. The protein acts as an antigen and reacts with an antibody, and then reacts with an enzyme or isotope-labeled secondary antibody , After the substrate color or autoradiography to detect the specific target gene expression protein components. This technique is also widely used to detect the expression of protein levels.
Of course, in addition to the detection of luminol ECL luminescence solution in WB experiment, other color development methods can also be used, such as the substrate DAB color development or the new Trinder's reagent enzyme-linked immunoassay, and Desheng has a variety of peroxidase luminescent substrates. Luminol and chromogenic substrate TOOS, MAOS, etc.

521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB 2

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