521-31-3 Chemiluminescent Substrate Luminol hrp In Western Blot WB

Application of chemiluminescent substrate luminol in western blot WB；CAS 521-31-3；Purity 98% ；Yellow powder

Application of chemiluminescent substrate luminol in western blot WB

Luminol, also called luminol, can detect a very small amount of hydrogen peroxide. It is usually used as a substrate for peroxidase for various biochemical detections. It is very sensitive and easy to automate. Among them, Western blot WB experiments are often used. Use ECL luminescent liquid made of luminol.

In Western Blot (Western Blot), that is, Western blotting, the ECL luminescent solution used is made of luminol. Luminol luminescence immunoassay is usually the last step of Western Blot, adding 1:1 solution A and solution B, covering the pvdf film, you can observe the experimental results by luminescence. The luminescent liquids A and B here are solutions made of luminol and hydrogen peroxide, respectively.

ECL luminous liquid raw material luminol luminol
Western blotting is a protein detection technique in which the total proteins of cells or tissues are separated by electrophoresis and transferred from the electrophoresis gel to a solid support NC membrane or PVDF membrane, and then a specific antibody is used to detect a specific antigen. It is now widely used It is used in many aspects such as gene expression research at the protein level, antibody activity detection and early disease diagnosis. After the target protein is labeled with HRP enzyme, it can be observed and detected by luminol luminescence.
Western Blot is similar to Southern Blot or Northern Blot, but the WB method uses polyacrylamide gel electrophoresis, and the object to be detected or blotted is protein. The protein or polypeptide sample is separated by PAGE and transferred to the carrier nitrocellulose membrane. The solid-phase carrier adsorbs the protein by non-covalent bonds and ensures its activity. The protein acts as an antigen and reacts with an antibody, and then reacts with an enzyme or isotope-labeled secondary antibody , After the substrate color or autoradiography to detect the specific target gene expression protein components. This technique is also widely used to detect the expression of protein levels.
Of course, in addition to the detection of luminol ECL luminescence solution in WB experiment, other color development methods can also be used, such as the substrate DAB color development or the new Trinder's reagent enzyme-linked immunoassay, and Desheng has a variety of peroxidase luminescent substrates. Luminol and chromogenic substrate TOOS, MAOS, etc.