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PH 11.5 Tris
The relationship between pH and temperature of tris(hydroxymethylaminomethane) and storage
The Chinese product names of Tris are tris(hydroxymethyl)aminomethane; tromethamine; tromethamine; 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.
Tris is used in the preparation of buffers in biochemistry and molecular biology experiments; pharmaceutical intermediates; used in the preparation of surfactants and vulcanization accelerators; preparation of water-soluble polymers containing Tris structural units as coatings Dispersant; preparation of indoor formaldehyde adsorption materials, etc.
Tris is often used as a biological buffer, and is often formulated with pH values of 6.8, 7.4, 8.0, and 8.8. Its structural formula and pH value vary greatly with temperature. Generally speaking, for every degree of temperature increase, the PH value drops by 0.03. Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments. For example, TAE and TBE buffers (used for the dissolution of nucleic acids) commonly used in biochemical experiments require Tris. Because it contains amino groups, it can undergo condensation reactions with aldehydes. Tris is a weak base. At room temperature (25°C, its pKa is 8.1; according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH of the aqueous solution of Tris base is around 10.5, and hydrochloric acid is generally added. Adjust the pH value to the required value to obtain the buffer solution of the pH value. But at the same time, pay attention to the influence of temperature on the pKa of Tris. Since the Tris buffer is a weakly alkaline solution, DNA will be removed in such a solution. Protonation, thereby improving its solubility. People often add EDTA to Tris hydrochloric acid buffer to make "TE buffer". TE buffer is used for DNA stabilization and storage. If the acid solution that adjusts the pH value is replaced with acetic acid , Then get "TAE buffer" (Tris/Acetate/EDTA), and change to boric acid to get "TBE buffer" (Tris/Borate/EDTA). These two buffers are usually used in nucleic acid electrophoresis experiments. By Tris TAE and TBE are the most commonly used reagents for DNA electrophoresis. TE (pH 8.0) is mainly used to dissolve DNA. (TE is the collective name of Tris plus EDTA.) 1MTris-HCl6.8 and 1.5MTris-HCl8.8 are The most commonly used reagent for SDS-PAGE.
Tris needs to be placed in a cool, dry and moisture-proof warehouse, and can be stored at room temperature. Pay attention to pest control.
The purity of Tris Hydroxymethylaminomethane developed and produced by Desheng is ≥99%, which has good water solubility and stable process. Packing specification: 25kg plastic drum for large package, 500ml bottle for small package.
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