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|Molecular Weight:||121.135||Molecular Formula:||C4H11NO3|
|Appearance:||White Crystal Powder||Storage Conditions:||Room Temperature, Light-proof And Moisture-proof|
99% 77-86-1 THAM
Factory supply Tris(hydroxymethyl)aminoethane/Tromethamine/Aminobutanol/THAM 99% 77-86-1
Advantages of Biological Buffer Tris
TRIS Chinese name: Tris (hydroxymethyl) aminomethane, white crystalline powder in appearance, good water solubility, can also be dissolved with ethyl acetate but the dissolution rate is relatively slow, not with ether, carbon tetrachloride, copper, aluminum There is a corrosive effect. Tris has a PKA value of 8 at 25°C and a buffer range of 7-9. Common Tris buffers include Tris-HCL buffer, Tris-phosphate buffer and a variety of derivative buffers, such as TBS, TE, etc. TRIS is a very important biological buffer, the CAS number is 77-86-1.
|PH value required(25℃)||7.10||7.20||7.30||7.40||7.50||7.60||7.70||7.80||7.90||8.00||8.10||8.20||8.30||8.40||8.50||8.60||8.70||8.80||8.90|
|0.1mol/L HCI volume||45.7||44.7||43.4||42||40.3||38.5||36.6||34.5||32||29.2||26.2||22.9||19.9||17.2||14.7||12.4||10.3||8.5||7|
In biochemical research, biological buffers are often used, and there are as many as 20-30 varieties of biological buffers. Biological buffers are widely used in the in vitro diagnostic industry and biochemical experiments. What are the advantages of Tris in biological buffers?
1. Tris has high solubility in water and is inert to the reaction of many enzymes.
2. Tris has a high buffering capacity. It is generally used to stabilize the pH of the reaction system. It has a strong buffering capacity between pH 7.5-9.0.
3. Tris is very alkaline, so this buffer system can be used to configure a wide range of pH buffers ranging from acidic to alkaline.
4. Tris has little interference to the biochemical process and will not react with calcium, magnesium ions and heavy metal ions.
5. Tris buffer is widely used, and plays an important role in the fields of biochemistry, cosmetics, in vitro diagnostics, molecular biology and coatings.
When using Tris buffer, it is also necessary to note that the pH value of Tris is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process. Tris will easily absorb CO2 in the air, and the prepared buffer should be tightly sealed to avoid loss. In addition, Tris will react with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals. It can also act with heavy metals, but it also has an inhibitory effect on some systems.
Tris has a certain interference effect on some PH electrodes, and it is necessary to use electrodes that are compatible with Tris solution. In addition, Tris is not suitable for the determination of bicinchoninic acid (BCA), and Tris needs to wear gloves and goggles during operation, because Tris is inhaled, ingestion and skin absorption have certain effects on the human body.
Desheng Biotechnology has been developing and producing biological buffers for many years, and has invested a lot of funds in the development and production of Tris series products. At present, the company's products have been sold to more than 100 countries around the world. The quality of the products is reliable, and the price can be negotiated. If you are interested, you can call for consultation. Desheng Biotechnology welcomes your calls.
Contact Person: Alexzhang