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CAS 77-86-1 Tromethamine TRIS,
101% Tromethamine TRIS,
CAS 77-86-1 tris hydrochloride
Some methods of TRIS use CAS.77-86-1 color white
TRIS, the full name of trishydroxymethylaminomethane, is a common biological buffer. Biological buffers are often used in biochemical research experiments and are of extremely important significance. They are a kind of conditioning fluid that can resist the influence of a small amount of strong acid and alkali from the outside and maintain the pH value basically unchanged. TRIS is not only widely used as a solvent for nucleic acids and proteins, but also one of the main components of protein electrophoresis buffers. It can also participate in the production of various chemical, pesticide, and pharmaceutical products. It is an important intermediate in the preparation of surfactants, vulcanization accelerators and some drugs. Things. In addition, how many people know about the application of TRIS in the extraction of plant protein?
Proteomics is to further understand the mechanism of life activities and the molecular mechanism of disease occurrence from the level of proteomics. In the research of proteomics, protein extraction is one of the most basic and critical links. TRIS is the extraction of plant protein. Commonly used reagents, methods include TRIS-HCl method, phenol method, TRIS-acetone-phenol method. This article will analyze and summarize these methods.
The TRIS-HCl method extracts the protein spectrum effect better than the TCA acetone method. The protein extracted by the TCA acetone method is unevenly distributed in the small molecular weight area, the protein spots are not clear, and the horizontal and vertical stripes are more serious. The TRIS-HCl method overcomes the above shortcomings and separates the acidity that is not easy to separate by the TCA acetone method. For protein, in addition to separating more medium molecular weight proteins, a lot of high molecular weight and low molecular weight proteins can also be obtained.
The phenol method uses the characteristics of TRIS-saturated phenol: Phenol is a good solvent for proteins. During the sample preparation process, proteins and lipids are dissolved in the phenol phase, and soluble substances such as salts, nucleic acids, polyphenols and polysaccharides enter the water phase; Centrifugal force and centrifugal time can separate the dense sugar to the upper layer of the supernatant as much as possible; the protein in the phenol phase is precipitated by the methanol solution of ammonium acetate, and then washed with cold acetone several times to remove pigments and ammonium ions. Impurities.
The TCA acetone method is combined with the phenol method for protein extraction, which combines the advantages of the TCA acetone precipitation method and the phenol method. First wash the remaining TCA with a methanol solution containing ammonium acetate and raise the pH of the solution above 7.0, and then use a mixture of phenol and SDS to dissolve the protein. The combined extraction of phenol and SDS is more effective than the extraction of phenol and SDS buffer alone. The TRIS-acetone-phenol method has achieved very good results in the protein extraction of many plants, and the background and bands of the electrophoretic pattern are very clear, and there are many protein spots. It can be widely used in the extraction of protein from the leaves and fruits of plants.
For the extraction of plant protein, not only the samples are diverse, but the ingredients contained in each plant are also different, and there are many interference factors, so there is no method that can be applied to various samples. In the experiment, we must first clarify the purpose, whether to obtain a target protein or all the proteins, and then select the extraction method according to the characteristics of the sample, and optimize it.
Hubei New Desheng Materials specializes in the production and development of biological buffers and blood collection tube additives, enzyme preparations, chemiluminescence reagents, luminescent substrates and other products. Desheng has been established for decades and has its own R&D team. It has been researching and developing biological buffers for many years. Its products include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, and PIPES. At present, the products produced by Desheng have been sold to many countries around the world, and they have been repurchased with praise, and have reached long-term cooperation with many companies. If you are interested in understanding, you can call for consultation. Desheng welcomes your calls.
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