Product Details
Place of Origin: EZHOU,CHINA
Brand Name: DESHENG
Certification: ISO9001:2008
Payment & Shipping Terms
Minimum Order Quantity: 10g
Price: Negotiable
Packaging Details: Plastic Bottle or Aluminium Film
Delivery Time: 1~3 DAYS AFTER RECEIVING PAYMENT
Payment Terms: T/T, L/C, Western Union,Palpay
Supply Ability: 100kg/month
Purity: |
99% Min |
Appreance: |
White Powder |
Water-soluble: |
Good |
Storage Condition: |
0-5℃ In Dry And Dark Place |
MW: |
331.36 |
Chemical Formula: |
C12H18NNaO4S.2H20 |
CAS#: |
82692-93-1 |
Purity: |
99% Min |
Appreance: |
White Powder |
Water-soluble: |
Good |
Storage Condition: |
0-5℃ In Dry And Dark Place |
MW: |
331.36 |
Chemical Formula: |
C12H18NNaO4S.2H20 |
CAS#: |
82692-93-1 |
TOOS, a chromogenic substrate, is a raw material used for color development in biochemical reagent kits. Its CAS number is 82692-96-4 and it can undergo a color reaction with 4-AAP catalyzed by hydrogen peroxide in the presence of peroxidase. The color reaction is sensitive and the detection is rapid. Here is the usage guide for TOOS reagents produced by our company, as shown below.
Product Name | TOOS,N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline |
Product appearance | Pure white crystalline powder |
Product Usage | Blood glucose, blood glucose test, liver function, liver function test kit, etc |
Product advantages | Purity above 99%, low impurities, stable production process |
Saving conditions | Sealed storage, avoiding light and moisture |
Origin | Ezhou, Hubei Province |
Operation process
Prepare reaction system: including TOOS, 4-AAP, POD, and other necessary reagents and buffers.
Add the test sample: Add the test sample to the reaction system to ensure thorough mixing between the sample and the reaction system.
Triggering reaction: Under appropriate conditions (such as specific temperature and time), substances in the reaction system undergo redox reactions.
Observing color development: As the reaction progresses, the generated red quinone imine compound will cause a change in the color of the solution.
TOOS powder
Result analysis
Measure the absorbance of the reaction solution at a specific wavelength (such as 590nm) using instruments such as a spectrophotometer.
By changing the absorbance value, the concentration of the target substance in the test sample can be calculated.
matters needing attention
Temperature is one of the important factors affecting the TOOS color reaction. Excessive or insufficient temperature can affect the reaction rate and product stability, therefore it is necessary to control the reaction within an appropriate temperature range (such as room temperature 20-25 ℃). Different detection items may require different reaction systems and conditions, so adjustments need to be made according to specific detection items in practical operations.
Save Suggestions
Although TOOS is stable at room temperature, in order to ensure its long-term stable storage, it is recommended to place it in a frozen environment (be careful not to freeze it to ice) and store it away from light. The TOOS reagent packaging box of Desheng is a foam insulation box, which contains an ice bag and contains a test report. Desheng usually uses a dark brown bottle with a good light proof container. The TOOS is white powder.
Ready to use, ready to use
When preparing TOOS as a working solution, it is recommended to follow the principle of "ready to use". This is because once the chromogenic substrate dissolves, especially in contact with air, it may gradually oxidize, leading to color changes and affecting the sensitivity and accuracy of detection. Therefore, it is advisable to minimize the preparation of solutions in advance and ensure that timely solutions are used in each experiment to obtain more accurate experimental results.
Product packaging
Having a TOOS user guide can help you avoid detours during the experimental process. The TOOS chromogenic substrate developed by Desheng is an indispensable raw material in biochemical detection, which can quickly detect target substances. You need to control the temperature when using it to ensure that the reaction system is suitable, and TOOS needs to be frozen and stored away from light. It should be prepared and used immediately to maintain its stability and accuracy. Regardless, Desheng will be a reliable choice for your experiment.